区分白塞氏病和特发性复发性口疮的新型血清标记物

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Mengya Zhu, Xinliang Mao, Xianqian Huang, Minzhi Gan, Keyue Zhang, Yong Chen
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引用次数: 0

摘要

背景:白塞氏病(BD)是一种罕见的复发性自身炎症性疾病,以全身性血管炎为特征,常表现为复发性口腔炎(RAS)。我们的目的是鉴定特异性血清蛋白,以区分 BD 和特发性 RAS:方法:采集 12 名 BD 患者、12 名特发性 RAS 患者和 21 名健康志愿者的外周血。对血清样本进行基于串联质谱标签的质谱分析。对差异表达蛋白(DEPs)进行了KEGG通路富集、基因本体(GO)和蛋白-蛋白相互作用(PPI)分析。在由 18 名 BD 患者、18 名特发性 RAS 患者和 18 名对照组组成的另一个队列中,利用 ELISA 验证了两种 BD 特异性 DEPs:结果:与RAS血清相比,BD血清中出现了242种DEPs。与健康对照组相比,49种蛋白质在BD血清中有差异表达,而在RAS血清中没有。KEGG通路和GO分析显示,BD和RAS血清中的DEPs具有相似的生物学功能和细胞分布,其特点是与调节血液凝固和免疫反应的通路显著相关。在比较 BD 和 RAS 的 DEPs 时,有几种角蛋白成为区分 RAS 和 BD 的标志物。我们还在 BD 患者中发现了多种 DEPs,但在 RAS 患者中却没有发现。PPI分析发现,脂蛋白代谢调节因子是枢纽蛋白,表明它们在BD病理学中可能起着至关重要的作用。此外,ELISA结果证实,与健康供体相比,BD患者(而非RAS患者)的LRG1和SOD3水平升高:我们的数据发现了能区分 BD 和 RAS 的新型血清蛋白,它们可能有助于 BD 的诊断和治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel Serum Markers that Distinguish Behcet's Disease from Idiopathic Recurrent Aphthous Stomatitis.

Background: Behcet's disease (BD) is a rare and recurrent autoinflammatory disorder characterized by systemic vasculitis, frequently manifested as recurrent aphthous stomatitis (RAS). We aim to identify specific serum proteins to discriminate between BD and idiopathicRAS.

Method: Peripheral blood was collected from 12 BD patients, 12 idiopathic RAS patients, and 21 healthy volunteers. The serum samples underwent Tandem Mass Tag-based mass spectrometry analysis. Differentially expressed proteins (DEPs) were identified for KEGG pathway enrichment, Gene Ontology (GO), and protein-protein interaction (PPI) analyses. ELISA was utilized to verify two BD-specific DEPs in another cohort consisting of 18 BD patients, 18 idiopathic RAS patients, and 18 controls.

Results: Compared with RAS serum, BD serum showed 242 DEPs. 49 proteins were differentially expressed in BD but not RAS serum compared to healthy controls. KEGG pathway and GO analyses revealed that DEPs in BD and RAS have similar biological functions and cellular distributions, featuring a significant association with pathways regulating blood coagulation and immune response. When comparing DEPs between BD and RAS, several keratins emerged as markers that distinguish RAS from BD. We also identified multiple DEPs in BD but not RAS patients. PPI analysis uncovered that lipoprotein metabolism regulators serve as hub proteins, indicating their potentially essential roles in BD pathology. In addition, ELISA results confirmed the elevated LRG1 and SOD3 levels in BD, but not RAS patients, compared to healthy donors.

Conclusion: Our data uncovered novel serum proteins that distinguish BD from RAS, which may potentially be useful in BD diagnosis and treatment.

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来源期刊
Immunological Investigations
Immunological Investigations 医学-免疫学
CiteScore
5.50
自引率
7.10%
发文量
49
审稿时长
3 months
期刊介绍: Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.
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