剂量-反应曲线以及 IC50 和 EC50 值的确定

IF 6.8 1区医学 Q1 CHEMISTRY, MEDICINAL

Journal of Medicinal Chemistry Pub Date : 2024-10-01 DOI:10.1021/acs.jmedchem.4c02052

Bharath Srinivasan, Matthew D. Lloyd

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引用次数: 0

摘要

图 1.原钒酸钠（169 nM 至 100 μM）抑制牛碱性磷酸酶的剂量-反应曲线示例。测定（200 μL）在 96 孔板中进行，pH 8.24，50 mM Tris-HCl，400 mM 甘露醇，6 mM MgCl2，40 μM 4-硝基苯磷酸底物，1.5%（v/v）二甲基亚砜，每次测定 20.2 纳克酶。在 405 纳米波长处监测吸光度的变化。(16) (A).剂量-反应曲线与 log10 [药物 (M)]（参数）。(B).用摩尔药物浓度绘制的相同数据（非参数）。确定的 log10 IC50 值为 -6.7087，对应的 IC50 为 1.95 × 10-7 M，h 系数为 0.91。数据为平均值 ± SD。本文引用了 16 篇其他出版物。本文尚未被其他出版物引用。

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Dose–Response Curves and the Determination of IC50 and EC50 Values

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Dose–Response Curves and the Determination of IC50 and EC50 Values

Figure 1. Example dose–response curves for the inhibition of bovine alkaline phosphatase by sodium orthovanadate (169 nM to 100 μM). Assays (200 μL) were conducted in a 96-well plate in 50 mM Tris-HCl, 400 mM mannitol, 6 mM MgCl₂, pH 8.24, 40 μM 4-nitrophenyl phosphate substrate, 1.5% (v/v) DMSO, and 20.2 ng of enzyme per assay. The change in absorbance was monitored at 405 nm. (16) (A). Dose–response curve plotted with log₁₀ [drug (M)] (parametric). (B). The same data plotted with molar drug concentrations (nonparametric). The determined log₁₀ IC₅₀ value is −6.7087, corresponding to an IC₅₀ of 1.95 × 10^–7 M and an h-coefficient of 0.91. Data are means ± SD. This article references 16 other publications. This article has not yet been cited by other publications.

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来源期刊

Journal of Medicinal Chemistry 医学-医药化学

CiteScore

4.00

自引率

11.00%

发文量

804

审稿时长

1.9 months

期刊介绍： The Journal of Medicinal Chemistry is a prestigious biweekly peer-reviewed publication that focuses on the multifaceted field of medicinal chemistry. Since its inception in 1959 as the Journal of Medicinal and Pharmaceutical Chemistry, it has evolved to become a cornerstone in the dissemination of research findings related to the design, synthesis, and development of therapeutic agents. The Journal of Medicinal Chemistry is recognized for its significant impact in the scientific community, as evidenced by its 2022 impact factor of 7.3. This metric reflects the journal's influence and the importance of its content in shaping the future of drug discovery and development. The journal serves as a vital resource for chemists, pharmacologists, and other researchers interested in the molecular mechanisms of drug action and the optimization of therapeutic compounds.