Fanzor2的结构揭示了TnpB超家族进化的奥秘

IF 12.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature Structural & Molecular Biology Pub Date : 2024-10-01 DOI:10.1038/s41594-024-01394-4

Richard D. Schargel, M. Zuhaib Qayyum, Ajay Singh Tanwar, Ravi C. Kalathur, Elizabeth H. Kellogg

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引用次数: 0

摘要

RNA 引导的内切酶曾被认为是原核生物独有的，最近在真核生物中被发现，并被称为 Fanzor。它们被分为两个支系：Fanzor1 和 Fanzor2。在这里，我们展示了棘阿米巴多瘤拟态病毒 Fanzor2 的冷冻电镜结构，揭示了其 ωRNA 结构、活性位点以及参与转座子邻接基序识别的特征。通过与 Fanzor1 和 TnpB 结构的比较，我们发现了不同的进化路径，从而加深了我们对 RNA 引导的内切酶的了解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily

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Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily

RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases. This study presents the cryo-electron microscopy structure of Fanzor2, showcasing its unique structural elements and nucleic acid interaction sites. A comparison to TnpB-related RNA-guided endonucleases highlights divergent evolutionary paths.

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来源期刊

Nature Structural & Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOPHYSICS

CiteScore

22.00

自引率

1.80%

发文量

160

审稿时长

3-8 weeks

期刊介绍： Nature Structural & Molecular Biology is a comprehensive platform that combines structural and molecular research. Our journal focuses on exploring the functional and mechanistic aspects of biological processes, emphasizing how molecular components collaborate to achieve a particular function. While structural data can shed light on these insights, our publication does not require them as a prerequisite.