{"title":"芦荟提取物和金黄色葡萄球菌培养上清液合成的 AgNPs 的抗菌功效","authors":"","doi":"10.1016/j.jksus.2024.103464","DOIUrl":null,"url":null,"abstract":"<div><div>Biological synthesis of silver nanoparticles (AgNPs) is an emerging method that avoids the need for costly equipment and hazardous chemicals. Therefore, in this study, <em>Aloe vera</em> (<em>AV</em>) extract and culture supernatant of <em>Staphylococcus aureus</em> (CS-<em>S. aureus</em>) were used to reduce and stabilise silver nanoparticles. The NPs were characterized using UV–vis spectrophotometry at 445 nm for <em>AV</em>-AgNP and 444.5 nm for CS-<em>S.aureus</em> AgNP, and the presence of silver was confirmed by energy dispersive X-ray (EDX). Furthermore, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the <em>AV</em>-CS of <em>S. aureus</em>-AgNPs had irregular and spherical shapes, with average sizes of 18.5 nm for <em>AvAgNPs</em> and 7.03 nm for CS-<em>S</em>. <em>aureus</em>-AgNPs. Additionally, the minimum inhibitory concentration (MIC) of both AgNPs showed a similar reaction. <em>K. pneumoniae</em>, <em>P. aeruginosa</em>, and <em>S. epidermidis</em> were more sensitive to the AgNPs compared to methicillin-resistant <em>Staphylococcus aureus</em> (MRSA). In conclusion, our study used a green method to synthesise silver nanoparticles using <em>AV</em> extract and CS of <em>S. aureus</em>. The NPs showed a remarkable antibacterial effect, especially CS-<em>S. aureus-</em>AgNP, which could have application as a treatment against both Gram-positive and Gram-negative bacteria.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibacterial Efficacy of AgNPs synthesized from Aloe vera extract and Staphylococcus aureus Culture Supernatant\",\"authors\":\"\",\"doi\":\"10.1016/j.jksus.2024.103464\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Biological synthesis of silver nanoparticles (AgNPs) is an emerging method that avoids the need for costly equipment and hazardous chemicals. Therefore, in this study, <em>Aloe vera</em> (<em>AV</em>) extract and culture supernatant of <em>Staphylococcus aureus</em> (CS-<em>S. aureus</em>) were used to reduce and stabilise silver nanoparticles. The NPs were characterized using UV–vis spectrophotometry at 445 nm for <em>AV</em>-AgNP and 444.5 nm for CS-<em>S.aureus</em> AgNP, and the presence of silver was confirmed by energy dispersive X-ray (EDX). Furthermore, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the <em>AV</em>-CS of <em>S. aureus</em>-AgNPs had irregular and spherical shapes, with average sizes of 18.5 nm for <em>AvAgNPs</em> and 7.03 nm for CS-<em>S</em>. <em>aureus</em>-AgNPs. Additionally, the minimum inhibitory concentration (MIC) of both AgNPs showed a similar reaction. <em>K. pneumoniae</em>, <em>P. aeruginosa</em>, and <em>S. epidermidis</em> were more sensitive to the AgNPs compared to methicillin-resistant <em>Staphylococcus aureus</em> (MRSA). In conclusion, our study used a green method to synthesise silver nanoparticles using <em>AV</em> extract and CS of <em>S. aureus</em>. The NPs showed a remarkable antibacterial effect, especially CS-<em>S. aureus-</em>AgNP, which could have application as a treatment against both Gram-positive and Gram-negative bacteria.</div></div>\",\"PeriodicalId\":16205,\"journal\":{\"name\":\"Journal of King Saud University - Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of King Saud University - Science\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1018364724003768\",\"RegionNum\":3,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of King Saud University - Science","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1018364724003768","RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Antibacterial Efficacy of AgNPs synthesized from Aloe vera extract and Staphylococcus aureus Culture Supernatant
Biological synthesis of silver nanoparticles (AgNPs) is an emerging method that avoids the need for costly equipment and hazardous chemicals. Therefore, in this study, Aloe vera (AV) extract and culture supernatant of Staphylococcus aureus (CS-S. aureus) were used to reduce and stabilise silver nanoparticles. The NPs were characterized using UV–vis spectrophotometry at 445 nm for AV-AgNP and 444.5 nm for CS-S.aureus AgNP, and the presence of silver was confirmed by energy dispersive X-ray (EDX). Furthermore, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the AV-CS of S. aureus-AgNPs had irregular and spherical shapes, with average sizes of 18.5 nm for AvAgNPs and 7.03 nm for CS-S. aureus-AgNPs. Additionally, the minimum inhibitory concentration (MIC) of both AgNPs showed a similar reaction. K. pneumoniae, P. aeruginosa, and S. epidermidis were more sensitive to the AgNPs compared to methicillin-resistant Staphylococcus aureus (MRSA). In conclusion, our study used a green method to synthesise silver nanoparticles using AV extract and CS of S. aureus. The NPs showed a remarkable antibacterial effect, especially CS-S. aureus-AgNP, which could have application as a treatment against both Gram-positive and Gram-negative bacteria.
期刊介绍:
Journal of King Saud University – Science is an official refereed publication of King Saud University and the publishing services is provided by Elsevier. It publishes peer-reviewed research articles in the fields of physics, astronomy, mathematics, statistics, chemistry, biochemistry, earth sciences, life and environmental sciences on the basis of scientific originality and interdisciplinary interest. It is devoted primarily to research papers but short communications, reviews and book reviews are also included. The editorial board and associated editors, composed of prominent scientists from around the world, are representative of the disciplines covered by the journal.