胎牛血清浓度对人类脱落牙齿干细胞的干性和神经元分化标记的影响

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2024-09-24 DOI:10.1016/j.tice.2024.102571

Mateus de Oliveira Lisboa , Ana Helena Selenko , Agner Henrique Dorigo Hochuli , Alexandra Cristina Senegaglia , Letícia Fracaro , Paulo Roberto Slud Brofman

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引用次数: 0

摘要

从废弃牙齿中提取的牙齿干细胞（DSCs）是一种非侵入性且符合道德规范的来源，由于其来源于外胚层，因此具有神经发生的潜力。与其他间充质基质细胞（MSCs）相比，来自人类脱落牙齿（SHED）的干细胞具有高分化潜能和相对不成熟性，因此特别有前景。由于CD56和CD271等标记物在神经发育和保持干性方面的作用，它们对鉴定治疗用间叶干细胞亚群至关重要。本研究探讨了胎牛血清（FBS）浓度如何影响SHED中CD56和CD271的表达，从而影响它们的干性和神经元分化潜能。研究人员从不同的供体中分离出SHED，对其进行培养，并使用CD14、CD19、CD29、CD34、CD45、CD73、CD90、CD105、CD56和CD271等标记物对其间叶干细胞特征进行鉴定。在不同的 FBS 条件（标准 15%、降低 1%、5% 和无 FBS）下培养 SHED 表明，较低的 FBS 浓度会增加 CD271 和 CD56 的表达，同时保持标准的间充质干细胞免疫表型。重要的是，即使在标准 FBS 浓度下扩增 SHED 后，也能诱导这些标记物的表达增强。这些发现表明，FBS浓度可以优化SHED的培养条件，提高它们在再生医学和组织工程应用中的适用性。

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The influence of fetal bovine serum concentration on stemness and neuronal differentiation markers in stem cells from human exfoliated deciduous teeth

Dental Stem Cells (DSCs) from discarded teeth are a non-invasive and ethically favorable source with the potential for neurogenesis due to their ectodermal origin. Stem cells from human exfoliated deciduous teeth (SHED) are particularly promising due to their high differentiation potential and relative immaturity compared to other Mesenchymal Stromal Cells (MSCs). Markers like CD56 and CD271 are critical in identifying MSC subpopulations for therapeutic applications because of their roles in neurodevelopment and maintaining stemness. This study investigates how fetal bovine serum (FBS) concentrations affect the expression of CD56 and CD271 in SHED, influencing their stemness and neuronal differentiation potential. SHEDs were isolated from various donors, cultured, and characterized for MSC traits using markers such as CD14, CD19, CD29, CD34, CD45, CD73, CD90, CD105, CD56, and CD271. Culturing SHED in different FBS conditions (standard 15 %, reduced 1 % and 5 %, and FBS-free) showed that lower FBS concentrations increase CD271 and CD56 expression while maintaining the standard MSC immunophenotype. Importantly, the enhanced expression of these markers can be induced even after SHEDs have been expanded in standard FBS concentrations. These findings suggest that FBS concentration can optimize SHED culture conditions, enhancing their suitability for regenerative medicine and tissue engineering applications.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.