Yu-Xiang Liao, Lu Liu, Yuan-Bo Fu, Peng Chen, Tao Zhang, Fan Zhang, Ya-Li Wen, Shao-Song Wang, Xin DU, Jun Yang, Jiang-Yan Wei, Qi An, Shu-Han Jia, Hui-Lin Liu, Bin Li
{"title":"艾灸对逼尿肌过度活动大鼠膀胱ATP敏感钾通道（KATP）的影响","authors":"Yu-Xiang Liao, Lu Liu, Yuan-Bo Fu, Peng Chen, Tao Zhang, Fan Zhang, Ya-Li Wen, Shao-Song Wang, Xin DU, Jun Yang, Jiang-Yan Wei, Qi An, Shu-Han Jia, Hui-Lin Liu, Bin Li","doi":"10.13702/j.1000-0607.20230086","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of ginger-salt-partitioned moxibustion on ATP-sensitive potassium (K<sub>ATP</sub>) channel of bladder in detrusor overactivity (DO) rats.</p><p><strong>Methods: </strong>Female SD rats were randomly divided into sham operation, model, moxibustion and antagonist groups (<i>n</i>=9 in each group). Thorax (T) 10 spinal cord transection was performed by surgery. Ginger-salt partitioned moxibustion was applied to \"Shenque\" (CV8) for 3 cones, once daily for 14 consecutive days. Rats of the antagonist group were intraperitoneally injected with K<sub>ATP</sub> channel specific antagonist glibenclamide (10 μg·kg<sup>-1</sup>·d<sup>-1</sup>) once daily for 14 consecutive days. Urodynamic tests were performed after treatment. The distribution and expression of K<sub>ATP</sub> channel tetrameric subunit (SUR2B) in the bladder of rats was observed by immunofluorescence. The protein and mRNA expression levels of SUR2B in bladder tissue were detected by Western blot and qPCR respectively.</p><p><strong>Results: </strong>Compared with the sham operation group, rats of the model group showed intensive and large phasic contractions of the detrusor during bladder filling, the frequency and amplitude of phasic contractions of the detrusor 5 min before leakage were significantly increased (<i>P</i><0.001)；the voiding threshold pressure was significantly decreased (<i>P</i><0.001)；the bladder perfusion volume was increased (<i>P</i><0.001)；the SUR2B protein and mRNA expression in bladder tissue were significantly reduced (<i>P</i><0.001). Compared with the model group and the antagonist group, the above-mentioned indicators in the moxibustion group were all reversed (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Ginger-salt partitioned moxibustion can reduce the frequency and amplitude of detrusor phase contraction during bladder filling and prolong the time of first phase contraction in DO rats, which may be associated with up-regulating the expression level of K<sub>ATP</sub> channel protein and mRNA, promoting the outflow of potassium ions, and inhibiting the inflow of calcium ions, thus improve the stability of detrusor during storage.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"49 8","pages":"814-820"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of moxibustion on ATP-sensitive potassium channel (K<sub>ATP</sub>) of bladder in detrusor overactivity rats.\",\"authors\":\"Yu-Xiang Liao, Lu Liu, Yuan-Bo Fu, Peng Chen, Tao Zhang, Fan Zhang, Ya-Li Wen, Shao-Song Wang, Xin DU, Jun Yang, Jiang-Yan Wei, Qi An, Shu-Han Jia, Hui-Lin Liu, Bin Li\",\"doi\":\"10.13702/j.1000-0607.20230086\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To observe the effect of ginger-salt-partitioned moxibustion on ATP-sensitive potassium (K<sub>ATP</sub>) channel of bladder in detrusor overactivity (DO) rats.</p><p><strong>Methods: </strong>Female SD rats were randomly divided into sham operation, model, moxibustion and antagonist groups (<i>n</i>=9 in each group). Thorax (T) 10 spinal cord transection was performed by surgery. Ginger-salt partitioned moxibustion was applied to \\\"Shenque\\\" (CV8) for 3 cones, once daily for 14 consecutive days. Rats of the antagonist group were intraperitoneally injected with K<sub>ATP</sub> channel specific antagonist glibenclamide (10 μg·kg<sup>-1</sup>·d<sup>-1</sup>) once daily for 14 consecutive days. Urodynamic tests were performed after treatment. The distribution and expression of K<sub>ATP</sub> channel tetrameric subunit (SUR2B) in the bladder of rats was observed by immunofluorescence. The protein and mRNA expression levels of SUR2B in bladder tissue were detected by Western blot and qPCR respectively.</p><p><strong>Results: </strong>Compared with the sham operation group, rats of the model group showed intensive and large phasic contractions of the detrusor during bladder filling, the frequency and amplitude of phasic contractions of the detrusor 5 min before leakage were significantly increased (<i>P</i><0.001)；the voiding threshold pressure was significantly decreased (<i>P</i><0.001)；the bladder perfusion volume was increased (<i>P</i><0.001)；the SUR2B protein and mRNA expression in bladder tissue were significantly reduced (<i>P</i><0.001). Compared with the model group and the antagonist group, the above-mentioned indicators in the moxibustion group were all reversed (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>Ginger-salt partitioned moxibustion can reduce the frequency and amplitude of detrusor phase contraction during bladder filling and prolong the time of first phase contraction in DO rats, which may be associated with up-regulating the expression level of K<sub>ATP</sub> channel protein and mRNA, promoting the outflow of potassium ions, and inhibiting the inflow of calcium ions, thus improve the stability of detrusor during storage.</p>\",\"PeriodicalId\":34919,\"journal\":{\"name\":\"针刺研究\",\"volume\":\"49 8\",\"pages\":\"814-820\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"针刺研究\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.13702/j.1000-0607.20230086\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"针刺研究","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13702/j.1000-0607.20230086","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Effect of moxibustion on ATP-sensitive potassium channel (KATP) of bladder in detrusor overactivity rats.
Objectives: To observe the effect of ginger-salt-partitioned moxibustion on ATP-sensitive potassium (KATP) channel of bladder in detrusor overactivity (DO) rats.
Methods: Female SD rats were randomly divided into sham operation, model, moxibustion and antagonist groups (n=9 in each group). Thorax (T) 10 spinal cord transection was performed by surgery. Ginger-salt partitioned moxibustion was applied to "Shenque" (CV8) for 3 cones, once daily for 14 consecutive days. Rats of the antagonist group were intraperitoneally injected with KATP channel specific antagonist glibenclamide (10 μg·kg-1·d-1) once daily for 14 consecutive days. Urodynamic tests were performed after treatment. The distribution and expression of KATP channel tetrameric subunit (SUR2B) in the bladder of rats was observed by immunofluorescence. The protein and mRNA expression levels of SUR2B in bladder tissue were detected by Western blot and qPCR respectively.
Results: Compared with the sham operation group, rats of the model group showed intensive and large phasic contractions of the detrusor during bladder filling, the frequency and amplitude of phasic contractions of the detrusor 5 min before leakage were significantly increased (P<0.001)；the voiding threshold pressure was significantly decreased (P<0.001)；the bladder perfusion volume was increased (P<0.001)；the SUR2B protein and mRNA expression in bladder tissue were significantly reduced (P<0.001). Compared with the model group and the antagonist group, the above-mentioned indicators in the moxibustion group were all reversed (P<0.01, P<0.001, P<0.05).
Conclusions: Ginger-salt partitioned moxibustion can reduce the frequency and amplitude of detrusor phase contraction during bladder filling and prolong the time of first phase contraction in DO rats, which may be associated with up-regulating the expression level of KATP channel protein and mRNA, promoting the outflow of potassium ions, and inhibiting the inflow of calcium ions, thus improve the stability of detrusor during storage.
期刊介绍：
Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture.
The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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