从新生啮齿动物大脑中获取少突胶质细胞并使其成熟的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-09-28 DOI:10.1016/j.xpro.2024.103327

Hanki Kim, Bum Jun Kim, Seungyon Koh, Hyo Jin Cho, Xuelian Jin, Byung Gon Kim, Jun Young Choi

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引用次数: 0

摘要

少突胶质细胞原代培养模型的产生涵盖了少突胶质细胞系的不同阶段，对于少突胶质细胞生理和病理生理学的体外研究至关重要。在此，我们提供了一种从新生啮齿动物大脑中生成少突胶质细胞的方案。我们描述了通过差速离心分离少突胶质细胞祖细胞（OPCs）、随后扩增、传代和分化的步骤。有关该方案使用和执行的完整细节，请参阅 Kim 等人的文章1。

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Protocol for the acquisition and maturation of oligodendrocytes from neonatal rodent brains.

The generation of an oligodendrocyte primary culture model encompassing the diverse stages of the lineage is essential for the in vitro research of oligodendrocyte physiology and pathophysiology. Here, we provide a protocol for generating oligodendrocytes from the neonatal rodent brain. We describe steps for isolating oligodendrocyte progenitor cells (OPCs) through differential centrifugation, their subsequent expansion, passaging, and differentiation. For complete details on the use and execution of this protocol, please refer to Kim et al.¹.

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