利用第三代纳米孔测序技术对蜜蜂血淋巴的病毒和细菌季节性感染模式的新认识。

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Cato Van Herzele, Sieglinde Coppens, Nick Vereecke, Sebastiaan Theuns, Dirk C de Graaf, Hans Nauwynck
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引用次数: 0

摘要

蜜蜂正在迅速减少,这对我们的环境和农业构成了重大威胁。这些重要的昆虫面临着一种疾病综合症,据信是由寄生虫、病毒、杀虫剂和营养缺乏共同造成的。然而,真正的病因仍然是个谜。由于传统的分析方法,我们仍然缺乏对蜜蜂病毒组和致病菌存在的全面了解。为了填补这一知识空白,我们采用第三代纳米孔元基因组测序法对蜜蜂血淋巴进行了近一年的病原体监测。这项研究为了解蜜蜂群落中细菌和病毒载量的变化提供了宝贵的见解。我们在蜜蜂血淋巴中发现了不同的病原体,而这些病原体并不包括在蜜蜂筛查中。这些病原体包括Apis mellifera丝状病毒、Apis rhabdovirus和各种细菌,如Frischella sp.和Arsenophonus sp.。我们的研究提出,跨代免疫引物可能在蜜蜂感染模式的形成中发挥作用。我们观察到病原体数量在春季明显增加,随后在夏季和秋季病原体数量明显减少。然而,某些病原体似乎能够躲避这种诱导效应,这使它们成为导致蜜蜂死亡的潜在因素,特别引人关注。今后,我们将扩大对蜜蜂跨代免疫启动的研究,并调查其在自然环境中的潜力。这些知识最终将提高蜜蜂的健康水平,降低蜂群死亡率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph.

Honey bees are rapidly declining, which poses a significant threat to our environment and agriculture industry. These vital insects face a disease complex believed to be caused by a combination of parasites, viruses, pesticides, and nutritional deficiencies. However, the real aetiology is still enigmatic. Due to the conventional analysis methods, we still lack complete insights into the honey bee virome and the presence of pathogenic bacteria. To fill this knowledge gap, we employed third-generation nanopore metagenomic sequencing on honey bee haemolymph to monitor the presence of pathogens over almost a year. This study provides valuable insights into the changes in bacterial and viral loads within honey bee colonies. We identified different pathogens in the honey bee haemolymph, which are not included in honey bee screenings. These pathogens comprise the Apis mellifera filamentous virus, Apis rhabdoviruses, and various bacteria such as Frischella sp. and Arsenophonus sp. Furthermore, a sharp contrast was observed between young and old bees. Our research proposes that transgenerational immune priming may play a role in shaping infection patterns in honey bees. We observed a significant increase in pathogen loads in the spring, followed by a notable decrease in pathogen presence during the summer and autumn months. However, certain pathogens seem to be able to evade this priming effect, making them particularly intriguing as potential factors contributing to mortality. In the future, we aim to expand our research on honey bee transgenerational immune priming and investigate its potential in natural settings. This knowledge will ultimately enhance honey bee health and decrease colony mortality.

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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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