评估在临界储存条件下真实病例死后血液样本中自发超时高铁血红蛋白的形成。

IF 3.9 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Toxics Pub Date : 2024-09-14 DOI:10.3390/toxics12090670
Sara Gariglio, Maria Chiara David, Alessandro Mattia, Francesca Consalvo, Matteo Scopetti, Martina Padovano, Stefano D'Errico, Donato Morena, Paola Frati, Alessandro Santurro, Vittorio Fineschi
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引用次数: 0

摘要

亚硝酸盐/硝酸盐中毒是一个新出现的问题,据报告,在一些国家,亚硝酸盐/硝酸盐中毒事件不断增加,而且中毒者有自杀倾向。亚硝酸盐的毒性主要是与血红蛋白(Hb)相互作用,使其氧化成高铁血红蛋白(MetHb)。为了支持对这些案例进行分析的正确程序,本研究旨在评估在法医毒理学实验室的典型储存条件下样本的自发降解及由此产生的高铁血红蛋白。本研究使用了两种不同类型的样本:第一阶段的研究包括对毒理学实验室已储存四年(2018-2021 年)的司法尸检获得的血液样本进行回顾性分析,而第二阶段使用的样本是在司法尸检期间临时收集的。对这些样本采用衍生分光光度法获得的数据表明,似乎不存在一个形成 MetHb 的最大阈值,在该阈值范围内,可以足够肯定地指出所发现的 MetHb 浓度符合死前形成的情况,而不是样本降解和储存条件造成的假象。另一方面,研究结果表明,MetHb 的形成取决于样本采集和分析之间的时间间隔,因此,在实验室收到样本后立即对样本进行临时处理,对于在需要进行 MetHb 定量时从数据中获得最大可靠性和诊断价值至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Spontaneous Overtime Methemoglobin Formation in Post-Mortem Blood Samples from Real Cases in Critical Storage Conditions.

Nitrite/nitrate poisoning is an emerging problem, with an ongoing escalation of reported self-administration with suicidal intent in several countries. Nitrites toxicity mainly consists of their interaction with hemoglobin (Hb), causing its oxidization to methemoglobin (MetHb). In order to give support to the correct procedures for the analysis of these cases, this study aims to evaluate spontaneous sample degradation and consequent MetHb formation in the typical storage conditions of a forensic toxicology laboratory. Two different types of samples have been used in this study: the first stage of our study consisted of a retrospective analysis of blood samples obtained by judicial autopsies already stored in the toxicology laboratory, collected over four years (2018-2021), while the samples used for the second stage were appositely collected during judicial autopsies. The data obtained by the application of a derivative spectrophotometry method on these samples suggest that there seems not to be a maximum threshold for MetHb formation within which it is possible to state with a sufficient grade of certainty that the concentration of MetHb found is consistent with an ante-mortem formation and is not the result of an artifact due to sample degradation and storage conditions. On the other hand, the results suggest that MetHb formation depends on the time passed between sample collection and analysis, so that a tempestive sample processing, performed as soon as the samples are received in the laboratory, is crucial to obtain the maximum reliability and diagnostic values from the data when MetHb quantitation is necessary.

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来源期刊
Toxics
Toxics Chemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍: The Journal accepts papers describing work that furthers our understanding of the exposure, effects, and risks of chemicals and materials in humans and the natural environment as well as approaches to assess and/or manage the toxicological and ecotoxicological risks of chemicals and materials. The journal covers a wide range of toxic substances, including metals, pesticides, pharmaceuticals, biocides, nanomaterials, and polymers such as micro- and mesoplastics. Toxics accepts papers covering: The occurrence, transport, and fate of chemicals and materials in different systems (e.g., food, air, water, soil); Exposure of humans and the environment to toxic chemicals and materials as well as modelling and experimental approaches for characterizing the exposure in, e.g., water, air, soil, food, and consumer products; Uptake, metabolism, and effects of chemicals and materials in a wide range of systems including in-vitro toxicological assays, aquatic and terrestrial organisms and ecosystems, model mammalian systems, and humans; Approaches to assess the risks of chemicals and materials to humans and the environment; Methodologies to eliminate or reduce the exposure of humans and the environment to toxic chemicals and materials.
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