5G 射频电磁辐射对体外暴露公猪精液活力指标和 DNA 完整性的影响。

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2024-09-25 DOI:10.1016/j.theriogenology.2024.09.025

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引用次数: 0

摘要

近年来，射频电磁辐射（RF-EMR）对精液质量的影响一直是人们关注的焦点，但迄今为止的研究结果却相互矛盾。射频电磁辐射的影响取决于频率等因素，目前还没有关于 5G 频率对男性 DNA 完整性和精子活力影响的文献。本研究的目的是调查 5G 射频-电磁波对精子膜完整性、线粒体电位和体外暴露的种公猪精液 DNA 完整性的影响。研究包括 8 头皮特兰种公猪和 4 头德国兰德种公猪的精液样本，它们的年龄在 1.5 至 3.5 岁之间。每头公猪的新鲜稀释精液分为对照组（n = 12）和实验组（n = 12）。实验组的样本在单一频率（700 MHz、2500 MHz 和 3500 MHz）、电磁场强度为 10 V/m 的横向千兆赫电磁池中连续暴露 2 小时。使用 Halomax® 试剂盒对精子 DNA 碎片进行评估，使用 PI⁄SYBR-14 LIVE⁄DEAD 活力试剂盒和 JC-1 对精子膜完整性和线粒体电位进行评估。与对照组相比，暴露精液样本中出现 DNA 断裂的精子比例明显较高。暴露于 2500 MHz 的 5G 射频-EMR 的精液样本的 DNA 损伤程度最高（p

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Effects of 5G radiofrequency electromagnetic radiation on indicators of vitality and DNA integrity of in vitro exposed boar semen

The effects of radiofrequency electromagnetic radiation (RF-EMR) on semen quality have been in the spotlight in recent years, though research results to date have been contradictory. The effects of RF-EMR amongst others depend upon frequency, and there is currently no literature concerning the influence of 5G frequencies on both DNA integrity and spermatozoa vitality in males. The aim of this study was to investigate the effect of 5G RF-EMR on sperm membrane integrity, mitochondrial potential, and DNA integrity of in vitro exposed semen of breeding boars. The study included semen samples of eight breeding boars of the Pietren breed and four breeding boars of the German Landrace breed, from 1.5 to 3.5 years in age. Freshly diluted semen of each boar was divided into a control (n = 12) and experimental group (n = 12). The samples of the experimental group were exposed for 2 hours to continuous RF-EMR at a single frequency (700 MHz, 2500 MHz and 3500 MHz) and an electromagnetic field strength of 10 V/m using a transverse gigahertz electromagnetic cell. Sperm DNA fragmentation was assessed using a Halomax® kit and sperm membrane integrity and mitochondrial potential was assessed using a PI⁄SYBR-14 LIVE⁄DEAD viability kit with JC-1. A significantly higher proportion of spermatozoa with DNA fragmentation was found in exposed semen samples for all frequencies compared to the control group. The highest DNA damage was recorded in semen samples exposed to 5G RF-EMR at 2500 MHz (p < 0.01) and 3500 MHz (p < 0.05) vs. control semen samples. A significantly higher proportion of spermatozoa with damaged cell membrane and good mitochondrial potential was recorded in semen samples exposed with 3500 MHz. In vitro exposure of breading boar semen to 5G RF-EMR significantly increases the proportion of DNA fragmentation. The harmful effect of 5G RF-EMR on the proportion of spermatozoa with damaged DNA was frequency dependent. The 3500 MHz frequency displayed the most harmful effects due to significant impacts on DNA integrity and spermatozoa vitality indicators.

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.