初步评估靶向 DNMT1 的小分子探针在检测宫颈癌 PAX1 甲基化方面的价值

IF 2 4区医学 Q3 DERMATOLOGY

Skin Research and Technology Pub Date : 2024-10-01 DOI:10.1111/srt.70074

Baohua Yang, Qunxian Cheng, Xiaoqian Hong, Xiuxiang Zhu, Ziyin Xia, Wei Chen, Ling Xu

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引用次数: 0

摘要

背景：研究一种评估宫颈癌 PAX1 甲基化的小分子探针的筛查价值：材料与方法：通过绘制受试者的接收者操作特征曲线（ROC），评估宫颈病变灰度值的诊断阈值：通过绘制受试者的接收者操作特征曲线（ROC）来评估宫颈病变灰度值的诊断阈值。四组受试者的灰度值存在明显差异（P 结果：四组受试者的灰度值均高于对照组（P灰度值的差异 ROC 曲线显示，探针对宫颈炎和低度鳞状上皮内病变（LSIL）的诊断曲线下面积为 0.8724（95% CI = 0.7762-0.9685，p 结论：小分子探针具有一定的应用价值：小分子探针在区分宫颈病变类型方面有一定的应用价值，在区分宫颈炎症和癌前病变与癌变方面有较好的疗效，但在确定癌前病变类型方面疗效较差。

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Preliminary Evaluation of the Value of a Small-Molecule Probe Targeting DNMT1 in Detecting the Methylation of PAX1 in Cervical Cancer.

Background: To investigate the screening value of a small-molecule probe to assess the methylation of PAX1 in cervical cancer.

Materials and methods: The diagnostic threshold of the grayscale values for cervical lesions was assessed by plotting the Receiver Operating Characteristic (ROC) curve of subjects. Grayscale values were significantly different among the four groups (p < 0.05). Compared with the LSIL and cervicitis groups, a considerably higher grayscale value was found in the CA and high-grade squamous intraepithelial lesion (HSIL) groups (both p < 0.05).

Results: The differential ROC curves of the grayscale values showed that the diagnostic Area Under Curve of the probe for cervicitis and low-grade squamous intraepithelial lesion (LSIL) was 0.8724 (95% CI = 0.7762-0.9685, p < 0.0001), for cervicitis and CA was 1.0000 (p < 0.0001), for the LSIL and HSIL was 0.5484 (95% CI = 0.3826-0.7142, p = 0.5755), and for the LSIL and CA was 0.7724 (95% CI = 0.6016-0.9432, p = 0.0138).

Conclusion: The small molecular probe has certain application value in differentiating the type of cervical lesions and has better efficacy in distinguishing cervical inflammatory and precancerous lesions from carcinogenesis, but less efficacy in determining the type of precancerous lesions.

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来源期刊

Skin Research and Technology 医学-皮肤病学

CiteScore

3.30

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.