噪音会促进阿尔茨海默病样病变和 DNA 损伤

IF 1.3 4区 医学 Q3 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY
Noise & Health Pub Date : 2024-07-01 Epub Date: 2024-09-30 DOI:10.4103/nah.nah_26_24
Xiao-Jie Dai, Jun-Hua Liao, Yi Jia, Rui Cao, Mei-Ning Zhou
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引用次数: 0

摘要

研究目的本研究旨在探讨噪声导致阿尔茨海默病(AD)样病变发生的机制:将雄性Wistar大鼠(24个月)分为两组(每组6只):噪音组每天8:00至12:00暴露于98分贝声压级的白噪音中4小时,为期30天;对照组不暴露于噪音。通过新物体识别和莫里斯水迷宫测试评估大鼠的认知功能。然后收集海马组织,并使用酶联免疫吸附试验(ELISA)测定淀粉样β 1-42(Aβ1-42)、Aβ1-40、脑源性神经营养因子(BDNF)和肌球蛋白受体激酶B(TrkB)的水平。蛋白质表达通过 Western 印迹进行评估:结果:暴露于噪声会明显损害认知和识别能力,增加逃逸潜伏期,减少穿越平台象限交叉点的次数和在目标象限停留的时间(P < 0.01)。噪音组大鼠的新物体探索和识别指数明显下降(P < 0.01)。ELISA结果显示,噪音组大鼠海马中Aβ1-40和Aβ1-42水平升高,BDNF和TrkB水平降低(P < 0.01)。Western印迹分析显示,噪声组大鼠海马中β位淀粉样前体蛋白(APP)裂解酶1、磷酸化tau蛋白、γ-H2A组蛋白家族成员X、检查点激酶2、p53和p21显著升高(P < 0.01):结论:长期暴露于噪声可导致老年大鼠海马基因损伤,从而导致认知障碍,并出现类似于老年痴呆症的病变。因此,噪声是神经退行性疾病的潜在风险因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Noise Exposure Promotes Alzheimer's Disease-Like Lesions and DNA Damage.

Objective: This study aimed to explore the mechanism by which noise contributes to the development of Alzheimer's disease (AD)-like lesions.

Method: Male Wistar rats (24 months) were allocated into two groups (n = 6 per groups): a noise group exposed to 98 dB sound pressure-level white noise for 4 hours daily from 8:00 to 12:00 for 30 days, and a control group without noise exposure. The cognitive functions of the rats were assessed using new-object recognition and Morris water maze tests. Then, hippocampal tissues were collected, and the levels of amyloid β 1-42 (Aβ1-42), Aβ1-40, brain-derived neurotrophic factor (BDNF), and tropomyosin receptor kinase B (TrkB) were measured using enzyme-linked immunosorbent assay (ELISA). Protein expression was evaluated through Western blot.

Results: Noise exposure significantly impaired cognitive and recognition abilities, increased the escape latency, and decreased the number of crossings through the platform quadrant intersection and the time spent in the target quadrant (P < 0.01). The new-object exploration and recognition index of the rats in the noise group markedly decreased (P < 0.01). ELISA results indicated increases in Aβ1-40 and Aβ1-42 levels and decreases in BDNF and TrkB levels in the rat hippocampus in the noise group (P < 0.01). Western blot analyses revealed that beta-site amyloid precursor protein (APP) cleaving enzyme 1, phosphorylated tau protein, gamma-H2A histone family, member X, checkpoint kinase 2, p53, and p21 were remarkably elevated in the noise group (P < 0.01).

Conclusion: Chronic noise exposure can cause hippocampal genetic damage in aged rats, leading to cognitive disorders and the development of lesions similar to those observed in AD. Thus, noise is a potential risk factor for neurodegenerative disorders.

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来源期刊
Noise & Health
Noise & Health AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY-PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
CiteScore
2.10
自引率
14.30%
发文量
27
审稿时长
6-12 weeks
期刊介绍: Noise and Health is the only International Journal devoted to research on all aspects of noise and its effects on human health. An inter-disciplinary journal for all professions concerned with auditory and non-auditory effects of occupational, environmental, and leisure noise. It aims to provide a forum for presentation of novel research material on a broad range of topics associated with noise pollution, its control and its detrimental effects on hearing and health. It will cover issues from basic experimental science through clinical evaluation and management, technical aspects of noise reduction systems and solutions to environmental issues relating to social and public health policy.
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