Jia-Jun Ren, Xiu-Wen Yuan, Zi-Long Meng, Neng-Hao Cao, Yong-Nan Xu, Nam-Hyung Kim, Ying-Hua Li
{"title":"香叶木素通过缓解氧化应激促进猪的早期胚胎发育","authors":"Jia-Jun Ren, Xiu-Wen Yuan, Zi-Long Meng, Neng-Hao Cao, Yong-Nan Xu, Nam-Hyung Kim, Ying-Hua Li","doi":"10.1002/mrd.23775","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various plants such as spearmint and spider moss, exhibits antioxidant, anti-inflammatory, and antiaging properties. Nonetheless, its impact on early embryonic development in pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation in an in vitro culture (IVC) medium on porcine embryo development and to elucidate the underlying mechanisms. Findings revealed that embryos cultured in IVC medium with 0.1 μM DIOS demonstrated an increased blastocyst formation rate, higher total cell number, reduced LC3B and CASPASE3 levels, elevated Nrf2 levels, decreased ROS, and enhanced GSH and mitochondrial membrane potential at the 4-cell embryonic stage. Additionally, the expression of proapoptotic genes (<i>CAS3</i>, <i>CAS8</i>, and <i>BAX</i>) and autophagy-related genes (<i>BECLIN1</i>, <i>ATG5</i>, <i>LC3B</i>, and <i>P62</i>) was downregulated, whereas the expression of embryonic development-related genes (<i>CDK1</i> and <i>CDK2</i>), antioxidant-related genes (<i>SOD1</i> and <i>SOD2</i>), and mitochondrial biogenesis-related genes (<i>NRF2</i>) was upregulated. These findings suggest that DIOS promotes early embryonic development in pigs by mitigating oxidative stress and enhancing mitochondrial function, thereby reducing autophagy and apoptosis levels.</p>\n </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 9","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress\",\"authors\":\"Jia-Jun Ren, Xiu-Wen Yuan, Zi-Long Meng, Neng-Hao Cao, Yong-Nan Xu, Nam-Hyung Kim, Ying-Hua Li\",\"doi\":\"10.1002/mrd.23775\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various plants such as spearmint and spider moss, exhibits antioxidant, anti-inflammatory, and antiaging properties. Nonetheless, its impact on early embryonic development in pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation in an in vitro culture (IVC) medium on porcine embryo development and to elucidate the underlying mechanisms. Findings revealed that embryos cultured in IVC medium with 0.1 μM DIOS demonstrated an increased blastocyst formation rate, higher total cell number, reduced LC3B and CASPASE3 levels, elevated Nrf2 levels, decreased ROS, and enhanced GSH and mitochondrial membrane potential at the 4-cell embryonic stage. Additionally, the expression of proapoptotic genes (<i>CAS3</i>, <i>CAS8</i>, and <i>BAX</i>) and autophagy-related genes (<i>BECLIN1</i>, <i>ATG5</i>, <i>LC3B</i>, and <i>P62</i>) was downregulated, whereas the expression of embryonic development-related genes (<i>CDK1</i> and <i>CDK2</i>), antioxidant-related genes (<i>SOD1</i> and <i>SOD2</i>), and mitochondrial biogenesis-related genes (<i>NRF2</i>) was upregulated. These findings suggest that DIOS promotes early embryonic development in pigs by mitigating oxidative stress and enhancing mitochondrial function, thereby reducing autophagy and apoptosis levels.</p>\\n </div>\",\"PeriodicalId\":18856,\"journal\":{\"name\":\"Molecular Reproduction and Development\",\"volume\":\"91 9\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Reproduction and Development\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mrd.23775\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Reproduction and Development","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mrd.23775","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress
Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various plants such as spearmint and spider moss, exhibits antioxidant, anti-inflammatory, and antiaging properties. Nonetheless, its impact on early embryonic development in pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation in an in vitro culture (IVC) medium on porcine embryo development and to elucidate the underlying mechanisms. Findings revealed that embryos cultured in IVC medium with 0.1 μM DIOS demonstrated an increased blastocyst formation rate, higher total cell number, reduced LC3B and CASPASE3 levels, elevated Nrf2 levels, decreased ROS, and enhanced GSH and mitochondrial membrane potential at the 4-cell embryonic stage. Additionally, the expression of proapoptotic genes (CAS3, CAS8, and BAX) and autophagy-related genes (BECLIN1, ATG5, LC3B, and P62) was downregulated, whereas the expression of embryonic development-related genes (CDK1 and CDK2), antioxidant-related genes (SOD1 and SOD2), and mitochondrial biogenesis-related genes (NRF2) was upregulated. These findings suggest that DIOS promotes early embryonic development in pigs by mitigating oxidative stress and enhancing mitochondrial function, thereby reducing autophagy and apoptosis levels.
期刊介绍：
Molecular Reproduction and Development takes an integrated, systems-biology approach to understand the dynamic continuum of cellular, reproductive, and developmental processes. This journal fosters dialogue among diverse disciplines through primary research communications and educational forums, with the philosophy that fundamental findings within the life sciences result from a convergence of disciplines.
Increasingly, readers of the Journal need to be informed of diverse, yet integrated, topics impinging on their areas of interest. This requires an expansion in thinking towards non-traditional, interdisciplinary experimental design and data analysis.
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