Abaasa Catherine N, Stange Claudia, Ayesiga Savino, Mulogo M Edgar, Kalyetsi Rogers, Lejju B Julius, Andama Morgan, Tamwesigire K Imelda, Bazira Joel, Byarugaba Frederick, Tiehm Andreas
{"title":"从乌干达姆巴拉拉不同水源分离的大肠杆菌的抗生素耐药性。","authors":"Abaasa Catherine N, Stange Claudia, Ayesiga Savino, Mulogo M Edgar, Kalyetsi Rogers, Lejju B Julius, Andama Morgan, Tamwesigire K Imelda, Bazira Joel, Byarugaba Frederick, Tiehm Andreas","doi":"10.2166/wh.2024.319","DOIUrl":null,"url":null,"abstract":"<p><p><i>Escherichia coli</i> is widely used as an indicator of recent faecal pollution of water. Most <i>E. coli</i> strains are commensals; however, isolates in water samples have been shown to carry antibiotic resistance determinants. In total, 47 <i>E. coli</i> were isolated from selected drinking water sources in Mbarara, Uganda. The isolates were examined for their susceptibility to seven antibiotics and the presence of nine antibiotic-resistance genes (mostly <i>β</i>-lactamase genes) and class 1 integrons. Isolates showed a high resistance to ampicillin of 55.5% and a high sensitivity to azithromycin and gentamicin at 98 and 96%, respectively. PCR analysis showed the presence of extended-spectrum <i>β</i>-lactamase genes <i>bla</i><sub>CTX-M-32</sub> and <i>bla</i><sub>CMY-2</sub> in 64 and 36% of the isolates. The carbapenemase genes <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>VIM-2</sub>, <i>bla</i><sub>NDM-1</sub>, and <i>bla</i><sub>KPC-3</sub> were either not detected or only in a very small number of the isolates, whereas class 1 integrons were present in 68% of the isolates. This study proves that antimicrobial resistance exists in <i>E. coli</i> in water used for drinking purposes in Mbarara city. There is a need for public health actors to improve the surveillance of microbiological quality of drinking water to minimize health risks.</p>","PeriodicalId":17436,"journal":{"name":"Journal of water and health","volume":"22 9","pages":"1579-1593"},"PeriodicalIF":2.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibiotic resistance of <i>E. coli</i> isolates from different water sources in Mbarara, Uganda.\",\"authors\":\"Abaasa Catherine N, Stange Claudia, Ayesiga Savino, Mulogo M Edgar, Kalyetsi Rogers, Lejju B Julius, Andama Morgan, Tamwesigire K Imelda, Bazira Joel, Byarugaba Frederick, Tiehm Andreas\",\"doi\":\"10.2166/wh.2024.319\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Escherichia coli</i> is widely used as an indicator of recent faecal pollution of water. Most <i>E. coli</i> strains are commensals; however, isolates in water samples have been shown to carry antibiotic resistance determinants. In total, 47 <i>E. coli</i> were isolated from selected drinking water sources in Mbarara, Uganda. The isolates were examined for their susceptibility to seven antibiotics and the presence of nine antibiotic-resistance genes (mostly <i>β</i>-lactamase genes) and class 1 integrons. Isolates showed a high resistance to ampicillin of 55.5% and a high sensitivity to azithromycin and gentamicin at 98 and 96%, respectively. PCR analysis showed the presence of extended-spectrum <i>β</i>-lactamase genes <i>bla</i><sub>CTX-M-32</sub> and <i>bla</i><sub>CMY-2</sub> in 64 and 36% of the isolates. The carbapenemase genes <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>VIM-2</sub>, <i>bla</i><sub>NDM-1</sub>, and <i>bla</i><sub>KPC-3</sub> were either not detected or only in a very small number of the isolates, whereas class 1 integrons were present in 68% of the isolates. This study proves that antimicrobial resistance exists in <i>E. coli</i> in water used for drinking purposes in Mbarara city. There is a need for public health actors to improve the surveillance of microbiological quality of drinking water to minimize health risks.</p>\",\"PeriodicalId\":17436,\"journal\":{\"name\":\"Journal of water and health\",\"volume\":\"22 9\",\"pages\":\"1579-1593\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of water and health\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.2166/wh.2024.319\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/22 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water and health","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2166/wh.2024.319","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/22 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Antibiotic resistance of E. coli isolates from different water sources in Mbarara, Uganda.
Escherichia coli is widely used as an indicator of recent faecal pollution of water. Most E. coli strains are commensals; however, isolates in water samples have been shown to carry antibiotic resistance determinants. In total, 47 E. coli were isolated from selected drinking water sources in Mbarara, Uganda. The isolates were examined for their susceptibility to seven antibiotics and the presence of nine antibiotic-resistance genes (mostly β-lactamase genes) and class 1 integrons. Isolates showed a high resistance to ampicillin of 55.5% and a high sensitivity to azithromycin and gentamicin at 98 and 96%, respectively. PCR analysis showed the presence of extended-spectrum β-lactamase genes blaCTX-M-32 and blaCMY-2 in 64 and 36% of the isolates. The carbapenemase genes blaOXA-48, blaVIM-2, blaNDM-1, and blaKPC-3 were either not detected or only in a very small number of the isolates, whereas class 1 integrons were present in 68% of the isolates. This study proves that antimicrobial resistance exists in E. coli in water used for drinking purposes in Mbarara city. There is a need for public health actors to improve the surveillance of microbiological quality of drinking water to minimize health risks.
期刊介绍：
Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.
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