费奇中片高效液相色谱指纹图谱的建立及十四种成分的同时测定

IF 2.3 3区 化学 Q3 CHEMISTRY, ANALYTICAL
Journal of Analytical Methods in Chemistry Pub Date : 2024-09-18 eCollection Date: 2024-01-01 DOI:10.1155/2024/7703951
Ting Yang, Fang Deng, Xuejun Yang, Shunxiang Li
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引用次数: 0

摘要

目的:建立飞蓟中片的高效液相色谱指纹图谱,同时测定飞蓟中片中的 14 种成分:采用 30°C 恒温 Shimadzu Shim-pack GIST-C18 色谱柱(250 mm × 4.6 mm, 5 um),以乙腈(A)-0.1%磷酸(B)为流动相,流速为 1.0 mL/min,梯度洗脱(0.1 mL/min-1.0 mL/min)。流动相为乙腈(A)-0.1%磷酸(B),流速为 1.0 mL/min,梯度洗脱(0-10 min,88%A;10-30 min,88%-50%A;30-60 min,50%-30%A;60-65 min,30%A;65-70 min,30%-88%A;70-75 min,88%A),检测波长为 246 nm:结果:20 批样品的高效液相色谱指纹图谱共有 115 个共同峰,相似度大于 0.953。其中14种主要活性成分在色谱图中的响应值较高,被选中进行定量分析,包括玉竹内酯B、丹参酮IIA、绿原酸、补骨脂苷、异补骨脂苷、冰片苷、苦杏仁苷、咖啡酸、花萼苷-7-O-β-D-葡萄糖苷、丹参酚酸B、槲皮素、补骨脂烯、异补骨脂烯和白屈菜醇。14种成分在各自范围内呈良好的线性关系(r ≥ 0.9985),平均回收率为90.71-106.14%,RSD值为0.23-1.22%:建立了一种快速、有效的飞蓟中片成分分离检测方法,为飞蓟中片的质量控制和定量检测提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment of HPLC Fingerprints for Feiqizhong Tablets and Simultaneous Determination of Fourteen Constituents.

Aim: To establish the HPLC fingerprints for Feiqizhong tablets and to simultaneously determine fourteen contents in Feiqizhong tablets.

Methods: The analysis of the methanol extract of this drug was performed on a 30°C thermostatic Shimadzu Shim-pack GIST-C18 column (250 mm × 4.6 mm, 5 um), with the mobile phase comprising acetonitrile (A) -0.1% phosphoric acid (B) flowing at 1.0 mL/min in a gradient elution manner (0-10 min, 88% A; 10-30 min, 88%-50% A; 30-60 min, 50%-30% A; 60-65 min, 30% A; 65-70 min, 30%-88% A; 70-75 min, 88% A), and the detection wavelength was fixed at 246 nm.

Results: There were one hundred and five common peaks in the HPLC fingerprints of twenty batches of samples with similarities of more than 0.953. Among them, fourteen major active components with a high response value in the chromatogram were selected for quantification, including eupalinolide B, tanshinone IIA, chlorogenic acid, psoralenoside, isopsoralenoside, icariin, amygdalin, caffeic acid, calycosin-7-O-β-D-glucoside, salvianolic acid B, quercetin, psoralen, isopsoralen, and bakuchiol. Fourteen constituents showed good linear relationships within their own ranges (r ≥ 0.9985), whose average recoveries were 90.71-106.14% with RSD values of 0.23-1.22%.

Conclusion: A rapid and effective method for the separation and detection of components in Feiqizhong tablets is established, which can provide a basis for quality control and quantitative detection.

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来源期刊
Journal of Analytical Methods in Chemistry
Journal of Analytical Methods in Chemistry CHEMISTRY, ANALYTICAL-ENGINEERING, CIVIL
CiteScore
4.80
自引率
3.80%
发文量
79
审稿时长
6-12 weeks
期刊介绍: Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical. Subject areas include (but are by no means limited to): Separation Spectroscopy Mass spectrometry Chromatography Analytical Sample Preparation Electrochemical analysis Hyphenated techniques Data processing As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
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