头状 RNA 通过与 CSDE1 和 hnRNP A3 相互作用促进蛇头瘤病毒（SHVV）的复制。

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2024-09-26 DOI:10.1016/j.fsi.2024.109930

Da Huang , Rui Cheng , Xiaodan Liu , JiaQi Zhang , Chi Zhang

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引用次数: 0

摘要

头绪RNA是病毒的小非编码RNA，已被证实在病毒复制中发挥重要作用。乌鳢囊状病毒（SHVV）是一种水生病毒，曾给中国乌鳢养殖业造成巨大的经济损失。研究证明，SHVV在感染过程中会产生头领RNA，头领RNA可与病毒核蛋白相互作用，促进病毒复制。在这项研究中，我们发现头领 RNA 还能与细胞蛋白 Cold Shock Domain containing E1（CSDE1）和异质核核糖核蛋白 A3（hnRNP A3）相互作用。进一步研究发现，过量表达 CSDE1 和 hnRNP A3 会促进 SHVV 复制。通过 siRNA 下调 CSDE1 和 hnRNP A3 可抑制 SHVV 复制。这项研究为了解 SHVV 复制机制提供了新的视角，并为药物研究提供了潜在的抗 SHVV 靶点。

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Leader RNA facilitates snakehead vesiculovirus (SHVV) replication by interacting with CSDE1 and hnRNP A3

Leader RNAs are viral small non-coding RNAs that has been proved to play important roles in viral replication. Snakehead vesiculovirus (SHVV) is an aquatic virus that has caused huge economic loss in Chinese snakehead fish aquaculture industry. It has been proved that SHVV would generate leader RNA during the process of infection, and leader RNA could interact with viral nucleoprotein to promote viral replication. In this study, we identified that leader RNA could also interact with cellular protein Cold Shock Domain containing E1 (CSDE1) and heterogeneous nuclear ribonucleoproteins A3 (hnRNP A3). Further investigation reveals that overexpression of CSDE1 and hnRNP A3 facilitated SHVV replication. Downregulation of CSDE1 and hnRNP A3 by siRNA inhibited SHVV replication. This study provided a new sight into understand the mechanism of SHVV replication, and a potential anti-SHVV target for drug research.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.