柚木提取物的抗氧化特性有助于尼古丁诱导的小鼠的神经化学和大脑结构调整

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Afaf A Alatawi, Jamaan S Ajarem, Saud A Alarifi, Saleh A Al-Quraishy, Esam M Al-Shaebi, Sarah A Alawwad, Rashed N Herqash, Sally M Khadrawy, Noha A Ahmed, Chuanyi Wang, Saleh N Maodaa
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引用次数: 0

摘要

介绍:吸烟是一个重大的全球性健康问题,与精神疾病和高死亡率有关。尼古丁是吸烟时吸收的主要化合物,会对多个器官造成伤害,尤其是大脑。本研究探讨了柚木提取物(TPE)对尼古丁诱导的小鼠大脑生化和组织学变化的调节作用:将 24 只小鼠分为四组,治疗三周。第一组为对照组,第二组口服 100 毫克/千克 TPE,第三组皮下注射 2.5 毫克/千克尼古丁,第四组同时服用尼古丁和 TPE:结果:尼古丁诱导组的脑组织出现组织病理学改变和氧化应激,表现为脂质过氧化和一氧化氮水平升高,同时谷胱甘肽含量和超氧化物歧化酶活性降低。彗星试验也检测到 DNA 断裂。使用 TPE 治疗后,氧化应激和 DNA 断裂明显减少,同时抗氧化生物标志物增加。组织病理学变化也有所减少:结论:通过TPE的抗氧化活性,它可以影响氧化应激、DNA碎片和脑组织病理学变化,从而保护小鼠免受尼古丁诱发的神经毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant Properties of Teucrium polium Extract Contribute to Neurochemical and Brain Structural Modulations in Nicotine-Induced Mice.

Introduction: Tobacco use is a major global health issue linked to psychiatric illnesses and high mortality rates. Nicotine, the primary compound absorbed during smoking, causes harm to various organs, particularly the brain. The current study examined the modulatory effect of Teucrium polium extract (TPE) on nicotine-induced biochemical and histological changes in the brains of mice.

Methods: Twenty-four mice were divided into four groups and were treated for three weeks. Group one was the control; Group two received 100 mg/kg TPE orally; Group three was subcutaneously injected with 2.5 mg/kg nicotine, and Group four received both nicotine and TPE.

Results: The brain tissue of the nicotine-induced group showed histopathological alterations and oxidative stress as indicated by increased lipid peroxidation and nitric oxide levels concomitant with decreased glutathione content and superoxide dismutase activity. DNA fragmentation was also detected by comet assay. Treatment with TPE significantly decreased oxidative stress and DNA fragmentation while increasing antioxidant biomarkers. Histopathological changes were also diminished.

Conclusion: Through the antioxidant activity of TPE, it protected against nicotine-induced neurotoxicity in mice by impacting oxidative stress, DNA fragmentation, and brain histopathological changes.

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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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