{"title":"Aphis gossypii Glover 中与发育期有关的 AgJHAMT 基因的功能分析。","authors":"Lianjun Zhang, Yuan Li, Xinhui Xu, Mengmeng Feng, Rukiya Turak, Xiaoning Liu, Hongsheng Pan","doi":"10.1017/S000748532400049X","DOIUrl":null,"url":null,"abstract":"<p><p><i>Aphis gossypii</i> is one of the most economically important agricultural pests that cause serious crop losses worldwide, and the indiscriminate chemical application causes resistance development in <i>A. gossypii,</i> a major obstacle to successful control. In this study, we selected the up-regulated expression gene <i>AgJHAMT</i>, which was enriched into juvenile hormone pathway though transcriptome sequencing analysis of the cotton aphids that fed on transgenic cotton lines expressing ds<i>AgCYP6CY3</i> (the TG cotton). The <i>AgJHAMT</i> gene was overexpressed in cotton aphids which fed on the TG cotton, and its expression profile during the nymphs was clarified. Then, silencing <i>AgJHAMT</i> could advance the developmental period of cotton aphids by 0.5 days compared with control groups. The <i>T</i> and <i>t</i> values of cotton aphids in the dsJHAMT treatment group (6.88 ± 0.15, 1.65 ± 0.06) were significantly shorter than that of the sprayed H<sub>2</sub>O control group (7.6 ± 0.14, 1.97 ± 0.09) (<i>P</i> < 0.05), respectively. The fast growth caused by <i>AgJHAMT</i> silencing was rescued by applying the JH analogue, methoprene. Overall, these findings clarified the function of <i>AgJHAMT</i> in the developmental period of <i>A. gossypii</i>. This study contributes to further clarify the molecular mechanisms of delaying the growth and development of cotton aphids by the transgenic cotton lines expressing ds<i>AgCYP6CY3</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"707-716"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functional analysis of <i>AgJHAMT</i> gene related to developmental period in <i>Aphis gossypii</i> Glover.\",\"authors\":\"Lianjun Zhang, Yuan Li, Xinhui Xu, Mengmeng Feng, Rukiya Turak, Xiaoning Liu, Hongsheng Pan\",\"doi\":\"10.1017/S000748532400049X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Aphis gossypii</i> is one of the most economically important agricultural pests that cause serious crop losses worldwide, and the indiscriminate chemical application causes resistance development in <i>A. gossypii,</i> a major obstacle to successful control. In this study, we selected the up-regulated expression gene <i>AgJHAMT</i>, which was enriched into juvenile hormone pathway though transcriptome sequencing analysis of the cotton aphids that fed on transgenic cotton lines expressing ds<i>AgCYP6CY3</i> (the TG cotton). The <i>AgJHAMT</i> gene was overexpressed in cotton aphids which fed on the TG cotton, and its expression profile during the nymphs was clarified. Then, silencing <i>AgJHAMT</i> could advance the developmental period of cotton aphids by 0.5 days compared with control groups. The <i>T</i> and <i>t</i> values of cotton aphids in the dsJHAMT treatment group (6.88 ± 0.15, 1.65 ± 0.06) were significantly shorter than that of the sprayed H<sub>2</sub>O control group (7.6 ± 0.14, 1.97 ± 0.09) (<i>P</i> < 0.05), respectively. The fast growth caused by <i>AgJHAMT</i> silencing was rescued by applying the JH analogue, methoprene. Overall, these findings clarified the function of <i>AgJHAMT</i> in the developmental period of <i>A. gossypii</i>. This study contributes to further clarify the molecular mechanisms of delaying the growth and development of cotton aphids by the transgenic cotton lines expressing ds<i>AgCYP6CY3</i>.</p>\",\"PeriodicalId\":9370,\"journal\":{\"name\":\"Bulletin of Entomological Research\",\"volume\":\" \",\"pages\":\"707-716\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Entomological Research\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1017/S000748532400049X\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1017/S000748532400049X","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Functional analysis of AgJHAMT gene related to developmental period in Aphis gossypii Glover.
Aphis gossypii is one of the most economically important agricultural pests that cause serious crop losses worldwide, and the indiscriminate chemical application causes resistance development in A. gossypii, a major obstacle to successful control. In this study, we selected the up-regulated expression gene AgJHAMT, which was enriched into juvenile hormone pathway though transcriptome sequencing analysis of the cotton aphids that fed on transgenic cotton lines expressing dsAgCYP6CY3 (the TG cotton). The AgJHAMT gene was overexpressed in cotton aphids which fed on the TG cotton, and its expression profile during the nymphs was clarified. Then, silencing AgJHAMT could advance the developmental period of cotton aphids by 0.5 days compared with control groups. The T and t values of cotton aphids in the dsJHAMT treatment group (6.88 ± 0.15, 1.65 ± 0.06) were significantly shorter than that of the sprayed H2O control group (7.6 ± 0.14, 1.97 ± 0.09) (P < 0.05), respectively. The fast growth caused by AgJHAMT silencing was rescued by applying the JH analogue, methoprene. Overall, these findings clarified the function of AgJHAMT in the developmental period of A. gossypii. This study contributes to further clarify the molecular mechanisms of delaying the growth and development of cotton aphids by the transgenic cotton lines expressing dsAgCYP6CY3.
期刊介绍：
Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.
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