用于检测溶液中蛋白质的原子力显微镜捕鱼技术。

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2024-09-01 DOI:10.18097/PBMC20247005273

T O Pleshakova, M O Ershova, A A Valueva, I A Ivanova, Yu D Ivanov, A I Archakov

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引用次数: 0

摘要

这篇综述探讨了在蛋白质浓度较低的溶液中使用原子力显微镜（AFM）作为蛋白质检测基本方法的可能性。新生物分析方法的需求是由用于蛋白质检测的常规系统灵敏度不足的问题决定的。本研究特别关注在生物分析中将原子力显微镜和捕鱼方法结合使用，作为一种将大体积分析溶液中的生物分子浓缩到小面积表面上的方法。

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AFM-fishing technology for protein detection in solutions.

The review considers the possibility of using atomic force microscopy (AFM) as a basic method for protein detection in solutions with low protein concentrations. The demand for new bioanalytical approaches is determined by the problem of insufficient sensitivity of systems used in routine practice for protein detection. Special attention is paid to demonstration of the use in bioanalysis of a combination of AFM and fishing methods as an approach of concentrating biomolecules from a large volume of the analyzed solution on a small surface area.

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).