XylR 调节 xyl 簇上的基因,参与 Herbaspirillum seropedicae Z69 中 D-木糖的分解代谢。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Ana Karen Malán, Juan José Marizcurrenaa, Manuela Oribe, Susana Castro-Sowinski, Silvia Batista
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引用次数: 0

摘要

木质纤维素生物质中最丰富的糖类之一 D-木糖并未被广泛用于生产具有附加值的生物产品,部分原因是缺乏能够有效代谢 D-木糖的工业微生物。Herbaspirillum seropedicae Z69 是一种 β-蛋白质细菌,能够积累聚-3-羟基丁酸,这是一种可生物降解的热塑性生物聚合物,含量超过 50%。它通过非磷酸化途径代谢 D-木糖。在 Z69 的基因组中,我们发现了 xylFGH(ABC D-木糖转运体)、xylB、xylD 和 xylC(高级非磷酸化途径)基因以及转录调节因子 xylR,从而形成了 xyl 簇。我们构建了基因敲除突变体 Z69ΔxylR,与 Z69 相比,该突变体在 D-木糖和 D-葡萄糖中的生长都有所降低。此外,我们还通过 RT-qPCR 和启动子融合分析了 xyl 基因的表达。这些结果表明,在 D-木糖存在的情况下,XylR 能激活木糖簇基因的表达。另一方面,XylR 并不调控 xylA、mhpD(低级非磷酸化途径)和 araB(L-阿拉伯糖脱氢酶)基因的表达。D-葡萄糖在这些基因调控机制中的参与仍有待阐明。这些结果有助于开发适应消耗木质纤维素糖的新菌株,以生产高附加值的生物产品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
XylR regulates genes at xyl cluster, involved in D-xylose catabolism in Herbaspirillum seropedicae Z69

D-xylose, one of the most abundant sugars in lignocellulosic biomass, is not widely used to produce bioproducts with added value, in part due to the absence of industrial microorganisms able to metabolize it efficiently. Herbaspirillum seropedicae Z69 is a β-proteobacterium able to accumulate poly-3-hydroxybutyrate, a biodegradable thermoplastic biopolymer, with contents higher than 50%. It metabolizes D-xylose by non-phosphorylative pathways. In the genome of Z69, we found the genes xylFGH (ABC D-xylose transporter), xylB, xylD, and xylC (superior non-phosphorylative pathway), and the transcriptional regulator xylR, forming the xyl cluster. We constructed the knock-out mutant Z69ΔxylR that has a reduced growth in D-xylose and in D-glucose, compared with Z69. In addition, we analyzed the expression of xyl genes by RT-qPCR and promoter fusion. These results suggest that XylR activates the expression of genes at the xyl cluster in the presence of D-xylose. On the other hand, XylR does not regulate the expression of xylA, mhpD (lower non-phosphorylative pathways) and araB (L-arabinose dehydrogenase) genes. The participation of D-glucose in the regulation mechanism of these genes must still be elucidated. These results contribute to the development of new strains adapted to consume lignocellulosic sugars for the production of value-added bioproducts.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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