揭示与越南中部高原地区自然发生的木犀属枯萎病和茎腐病有关的霉菌。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Dung Le
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引用次数: 0

摘要

枯萎病和茎腐病(WSR)是一种新出现的综合症,威胁着越南林同省的桔梗(Eustoma russellianum)切花生产。在林同省主要的桔梗种植区,包括 Da Lat、Lac Duong、Don Duong 和 Duc Trong,所有 13 个受检的商业桔梗温室中都观察到了这种病害,发病率随着植株年龄的增长而增加,从 7.5%到 32.4%不等。受感染的植株表现出发育不良、枯萎、茎腐、枯萎和倒伏,其中以枯萎和茎腐为主。病害表现为多环性,幼株的症状从随机或分散到最初剪花后的群集模式。根据形态学观察以及内部转录间隔区(ITS)和翻译伸长因子 1-α(TEF-1α)基因的系统发育分析,从染病的桔梗植株中分离出的 41 个类镰孢真菌被鉴定为 Fusarium vanleeuwenii(28 个分离株)、Neocosmospora solani(11 个分离株)和 F. annulatum(2 个分离株)。不同地点的镰刀菌物种组成各不相同,其中始终存在万袖镰刀菌。致病性试验证实,F. vanleeuwenii Li-Fo9511 、N. solani Li-Fs4311 和 F. annulatum Li-Fp3051 的分离物在体外试验中会引起典型的茎腐病。植物体内试验显示,秧苗在感染后两周开始枯萎,五到六周之间发病率和严重程度显著增加,尤其是 F. vanleeuwenii Li-Fo9511 。病原体经过重新分离和形态学确认,符合科赫推测。这是越南首次报告 F.vanleeuwenii、N. solani 和 F. annulatum 是连翘 WSR 的病原体,突出了有效控制策略的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam

Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam

Wilt and stem rot (WSR) is an emerging syndrome threatening cut lisianthus (Eustoma russellianum) production in Lam Dong province, Vietnam. The disease was observed in all 13 inspected commercial lisianthus greenhouses across major lisianthus cultivation areas in Lam Dong, including Da Lat, Lac Duong, Don Duong, and Duc Trong, with incidence increasing with plant age, ranging from 7.5 to 32.4%. Infected plants displayed stunting, wilting, stem rot and blight, and dieback, with predominance of wilt and stem rot. The disease showed polycyclic behavior, with symptoms shifting from random or scattered in young plants to clustered patterns after the initial flower cutting. Forty-one Fusaria-like fungal isolates recovered from diseased lisianthus plants were identified as Fusarium vanleeuwenii (28 isolates), Neocosmospora solani (11 isolates), and F. annulatum (2 isolates) based on morphological observations and phylogenetic analysis of the internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF-1α) genes. The composition of Fusaria species varied across sites, with F. vanleeuwenii being consistently present. Pathogenicity tests confirmed that isolates of F. vanleeuwenii Li-Fo9511, N. solani Li-Fs4311, and F. annulatum Li-Fp3051 caused typical stem rot in in-vitro assays. In-planta assays showed wilting in seedlings starting two weeks post-infection, with a remarkable increase in disease incidence and severity between five and six weeks, particularly for F. vanleeuwenii Li-Fo9511. The pathogens were re-isolated and morphologically confirmed, fulfilling Koch’s postulates. This is the first report of F. vanleeuwenii, N. solani, and F. annulatum as pathogens of lisianthus WSR in Vietnam, highlighting the need for effective control strategies.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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