不同叙利亚葡萄树和橄榄叶栽培品种酒精提取物抗氧化活性和光保护作用的比较研究

IF 2.1 Q3 PHARMACOLOGY & PHARMACY
Advances in Pharmacological and Pharmaceutical Sciences Pub Date : 2024-09-20 eCollection Date: 2024-01-01 DOI:10.1155/2024/7027281
Farah Alhakim, Antoun Laham, Jameela Hasian
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引用次数: 0

摘要

使用合成防晒霜的负面影响证明,需要一种由植物提取物制成的更安全的替代品。据报道,酚含量高的植物具有抗氧化特性。本研究的目的是确定在不同提取条件下从葡萄和橄榄叶中提取的乙醇提取物的酚含量和抗氧化特性。提取物采用 HPLC 进行定性和定量分析。以 80% 的乙醇(v/v)为溶剂进行索氏提取,结果令人满意。确定了葡萄叶提取物中的四种成分和橄榄叶提取物中的一种成分。使用 Folin-Ciocalteu 试剂测定的样品总酚含量(TPC)以没食子酸当量(GAEs)表示,橄榄干提取物为 38.39 至 72.78 毫克/克,葡萄干提取物为 65.918 至 132.7 毫克/克。Salmoni(葡萄叶)乙醇提取物的 TPC 最高(132 毫克 GAE/克),而 Zaity(橄榄叶)乙醇提取物的 TPC 最低(72.66 ± 0.46 GAE/克)。Folin-Ciocalteu 方法证明了植物中抗氧化剂的存在。通过清除 DPPH(2,2-二苯基-1-苦基肼)等自由基,测定了植物提取物的抗氧化能力。接着,分析了不同品种葡萄和橄榄叶的乙醇提取物,以确定其防晒系数(SPF)值。葡萄叶提取物和橄榄叶提取物的防晒系数分别为 28.8 和 29.96。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Study of Alcoholic Extracts of Different Syrian Grapevine and Olive Leaf Cultivars for Their Antioxidant Activity and Photoprotective Effects.

A safer alternative made of plant extracts is needed, as evidenced by the negative effects of using synthetic sunscreen. Antioxidant properties of plants with high phenolic content have been reported. The goal of this research was to ascertain the phenolic content and antioxidant characteristics of ethanolic extracts made from grape and olive leaves under various extraction settings. The extracts were subjected to both qualitative and quantitative analyses using HPLC. Soxhlet extraction with 80% ethanol (v/v) as the solvent produced a result that was satisfactory. Four components of grape leaf extract and one component of olive leaf extract were identified. Expressed as gallic acid equivalents (GAEs), the total phenolic content (TPC) of the samples, as determined by using the Folin-Ciocalteu's reagent, ranged from 38.39 to 72.78 mg/g dry extract of olive and 65.918-132.7 mg/g dry extract of grape. An ethanolic extract of Salmoni (grape leaves) had the highest TPC (132 mg GAE/g), while an ethanolic extract of Zaity (olive leaves) had the lowest (72.66 ± 0.46 GAE/g). The Folin-Ciocalteu method proved the existence of antioxidants in the plant. By scavenging free radicals such as DPPH (2,2-diphenyl-1-picrylhydrazyl), the antioxidant capacity of the plant extracts was determined. Next, the ethanolic extracts of various cultivars of grape and olive leaves were analyzed to determine their sun protection factor (SPF) value. It was 28.8 and 29.96 for grape leaf extract and olive leaf extract, respectively.

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来源期刊
CiteScore
4.30
自引率
3.60%
发文量
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审稿时长
17 weeks
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