{"title":"用于有毒物质降解和糖酸生产的一锅酶级联法。","authors":"Vinutsada Pongsupasa, Pangrum Punthong, Pimchai Chaiyen, Thanyaporn Wongnate","doi":"10.1002/cbic.202400281","DOIUrl":null,"url":null,"abstract":"<p>This study introduces a novel one-pot enzymatic cascade approach for converting toxicants and continuously generating an electron acceptor for production of sugar acids. This method offers a promising solution to concerns about pesticide toxicity and environmental contamination by transforming hazardous substances into a useful electron acceptor. This acceptor is then utilized to produce valuable chemicals with broad industrial applications, particularly in the food and pharmaceutical sectors. The cascade reaction employs organophosphate hydrolase (OPD) to convert pesticides into 4-nitrophenol (4-NP), which is subsequently transformed into 1,4-benzoquinone by HadA monooxygenase (HadA). 1,4-benzoquinone serves as an electron acceptor in the catalysis of sugar acid formation via pyranose dehydrogenase (PDH). The results indicate that this cascade reaction effectively converts lactose to lactobionic acid and xylose to 2-keto-xylonic acid. The latter can be further processed into xylonic acid through NaBH<sub>4</sub> reduction. Notably, the one-pot reaction yields up to 10 % higher compared to the direct addition of 1,4-benzoquinone. The synthesized xylonic acid exhibits exceptional water uptake properties in hydrogels, and the synthesized lactobionic acid shows antioxidant activity comparable to well-established antioxidants. These findings demonstrate the technological viability of these reaction cascades for various applications.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"25 23","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"One-Pot Enzymatic Cascade for Toxicant Degradation and Sugar Acid Production\",\"authors\":\"Vinutsada Pongsupasa, Pangrum Punthong, Pimchai Chaiyen, Thanyaporn Wongnate\",\"doi\":\"10.1002/cbic.202400281\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study introduces a novel one-pot enzymatic cascade approach for converting toxicants and continuously generating an electron acceptor for production of sugar acids. This method offers a promising solution to concerns about pesticide toxicity and environmental contamination by transforming hazardous substances into a useful electron acceptor. This acceptor is then utilized to produce valuable chemicals with broad industrial applications, particularly in the food and pharmaceutical sectors. The cascade reaction employs organophosphate hydrolase (OPD) to convert pesticides into 4-nitrophenol (4-NP), which is subsequently transformed into 1,4-benzoquinone by HadA monooxygenase (HadA). 1,4-benzoquinone serves as an electron acceptor in the catalysis of sugar acid formation via pyranose dehydrogenase (PDH). The results indicate that this cascade reaction effectively converts lactose to lactobionic acid and xylose to 2-keto-xylonic acid. The latter can be further processed into xylonic acid through NaBH<sub>4</sub> reduction. Notably, the one-pot reaction yields up to 10 % higher compared to the direct addition of 1,4-benzoquinone. The synthesized xylonic acid exhibits exceptional water uptake properties in hydrogels, and the synthesized lactobionic acid shows antioxidant activity comparable to well-established antioxidants. These findings demonstrate the technological viability of these reaction cascades for various applications.</p>\",\"PeriodicalId\":140,\"journal\":{\"name\":\"ChemBioChem\",\"volume\":\"25 23\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-09-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemBioChem\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cbic.202400281\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cbic.202400281","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
One-Pot Enzymatic Cascade for Toxicant Degradation and Sugar Acid Production
This study introduces a novel one-pot enzymatic cascade approach for converting toxicants and continuously generating an electron acceptor for production of sugar acids. This method offers a promising solution to concerns about pesticide toxicity and environmental contamination by transforming hazardous substances into a useful electron acceptor. This acceptor is then utilized to produce valuable chemicals with broad industrial applications, particularly in the food and pharmaceutical sectors. The cascade reaction employs organophosphate hydrolase (OPD) to convert pesticides into 4-nitrophenol (4-NP), which is subsequently transformed into 1,4-benzoquinone by HadA monooxygenase (HadA). 1,4-benzoquinone serves as an electron acceptor in the catalysis of sugar acid formation via pyranose dehydrogenase (PDH). The results indicate that this cascade reaction effectively converts lactose to lactobionic acid and xylose to 2-keto-xylonic acid. The latter can be further processed into xylonic acid through NaBH4 reduction. Notably, the one-pot reaction yields up to 10 % higher compared to the direct addition of 1,4-benzoquinone. The synthesized xylonic acid exhibits exceptional water uptake properties in hydrogels, and the synthesized lactobionic acid shows antioxidant activity comparable to well-established antioxidants. These findings demonstrate the technological viability of these reaction cascades for various applications.
期刊介绍:
ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).