开发用于器官特异性肝损伤评估的模块化 miRNA 反应生物传感器

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Xinxin Zhang, Tingting Wang, Xiangqing Fan, Meixia Wang, Zhixi Duan, Fang He, Hong-Hui Wang, Zhihong Li
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引用次数: 0

摘要

微小核糖核酸(miRNA)越来越被认为是多种疾病的重要诊断生物标志物和治疗靶标。近年来,研究人员一直强调需要开发能利用细胞外 miRNA 作为疾病诊断输入信号的探针。在这项研究中,我们介绍了一种新型的 miRNA 响应式生物传感器(miR-RBS),旨在实现高灵敏度和特异性的 miRNA 检测,尤其侧重于靶向器官特异性可视化。miR-RBS 采用了一种 Y 型结构的三链 DNA 探针(Y-TSDP),在正常生理条件下呈荧光淬灭状态。在高浓度 miRNA 存在的情况下,探针会切换到激活状态并发出荧光信号,从而实现快速准确的疾病报告。此外,miR-RBS 探针采用模块化设计,荧光标记链配备了一个功能模块,有助于与表达高水平目标受体的器官特异性结合。这样就可以利用aptameric锚定制miRNA检测和细胞靶向。研究结果表明,在药物诱导的肝损伤模型中,miR-RBS 探针能有效地观察到 miR-122 的水平,这表明它在疾病诊断和器官特异性成像方面具有良好的潜力。总之,这种创新的生物传感器为通过基于 miRNA 的生物标志物早期检测和监测疾病提供了一种多功能工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of a Modular miRNA-Responsive Biosensor for Organ-Specific Evaluation of Liver Injury.

MicroRNAs (miRNAs) are increasingly being considered essential diagnostic biomarkers and therapeutic targets for multiple diseases. In recent years, researchers have emphasized the need to develop probes that can harness extracellular miRNAs as input signals for disease diagnostics. In this study, we introduce a novel miRNA-responsive biosensor (miR-RBS) designed to achieve highly sensitive and specific detection of miRNAs, with a particular focus on targeted organ-specific visualization. The miR-RBS employs a Y-structured triple-stranded DNA probe (Y-TSDP) that exhibits a fluorescence-quenched state under normal physiological conditions. The probe switches to an activated state with fluorescence signals in the presence of high miRNA concentrations, enabling rapid and accurate disease reporting. Moreover, the miR-RBS probe had a modular design, with a fluorescence-labeled strand equipped with a functional module that facilitates specific binding to organs that express high levels of the target receptors. This allowed the customization of miRNA detection and cell targeting using aptameric anchors. In a drug-induced liver injury model, the results demonstrate that the miR-RBS probe effectively visualized miR-122 levels, suggesting it has good potential for disease diagnosis and organ-specific imaging. Together, this innovative biosensor provides a versatile tool for the early detection and monitoring of diseases through miRNA-based biomarkers.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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