开辟新天地：揭示血管钙化中的 USP1/ID3/E12/P21 轴。

IF 6.4 2区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Translational Research Pub Date : 2025-02-01 DOI:10.1016/j.trsl.2024.09.002

Aoran Huang , Jianyun Rao , Xin Feng , Xingru Li , Tianhua Xu , Li Yao

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引用次数: 0

摘要

血管钙化（VC）给心血管健康带来了重大挑战。本研究采用单细胞转录组测序技术来剖析这一过程中的细胞动态。我们发现了不同的细胞亚群，尤其是血管平滑肌细胞（VSMC），并观察到动脉粥样硬化钙化核心与邻近区域之间的差异。进一步研究发现，ID3 是调节 VSMC 功能的关键基因。体外实验证明，ID3 与 USP1 和 E12 相互作用，调节细胞增殖和成骨分化。动物模型证实了 USP1/ID3/E12/P21 轴在 VC 中的关键作用。这项研究揭示了一种新的调控机制，提供了潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Breaking new ground: Unraveling the USP1/ID3/E12/P21 axis in vascular calcification

Vascular calcification (VC) poses significant challenges in cardiovascular health. This study employs single-cell transcriptome sequencing to dissect cellular dynamics in this process. We identify distinct cell subgroups, notably in vascular smooth muscle cells (VSMCs), and observe differences between calcified atherosclerotic cores and adjacent regions. Further exploration reveals ID3 as a key gene regulating VSMC function. In vitro experiments demonstrate ID3′s interaction with USP1 and E12, modulating cell proliferation and osteogenic differentiation. Animal models confirm the critical role of the USP1/ID3/E12/P21 axis in VC. This study sheds light on a novel regulatory mechanism, offering potential therapeutic targets.

1.
This study uses single-cell transcriptome to investigate vascular calcification in depth.
2.
This study successfully identifies cellular heterogeneity related to vascular calcification.
3.
This study reveals the crucial role of ID3 in vascular smooth muscle cell osteogenic differentiation.
4.
In vivo and in vitro experiments of this study demonstrate the regulatory role of the USP1/ID3/E12/P21 axis in calcification.
5.
This study provides potential molecular targets for the treatment of calcification-related diseases.

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来源期刊

Translational Research 医学-医学：内科

CiteScore

15.70

自引率

0.00%

发文量

195

审稿时长

14 days

期刊介绍： Translational Research (formerly The Journal of Laboratory and Clinical Medicine) delivers original investigations in the broad fields of laboratory, clinical, and public health research. Published monthly since 1915, it keeps readers up-to-date on significant biomedical research from all subspecialties of medicine.