红细胞保存后期 miRNAs 的差异表达谱及其潜在作用。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-26 DOI:10.1016/j.tracli.2024.09.003

Yajie Wang , Yiming Ma , Liping Sun , Quan Rao , Xiaozhou Yuan , Yan Chen , Xiaofei Li

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引用次数: 0

摘要

目的检测红细胞保存后期不同表达的调控miRNA，并预测其作用：方法：收集保存期分别为 35 天、42 天和 50 天的悬浮红细胞，进行血常规检查、RNA 提取和小 RNA 测序文库制备。对构建的文库进行测序，并通过生物信息学分析后期储存的 RBC 中不同 miRNA 的生物学功能：结果：晚期红细胞的常规指标不受保存时间的明显影响。对不同保存天数的 RBC miRNA 进行的皮尔逊相关分析表明，RBC miRNA 随保存天数的增加而变化。第 35 天（D35）、第 42 天（D42）和第 50 天（D50）的 RBC miRNA 有显著差异（PConclusion：在 RBC 保存后期，miRNAs 有不同程度的表达，它们可能参与了各种生物学过程，尤其是癌症。

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Profiles of differential expression of miRNAs in the late stage of red blood cell preservation and their potential roles

Objective

To detect the differentially expressed regulatory miRNAs in the late stage of red blood cell (RBC) preservation and predict their roles.

Methods

Suspended RBCs with different storage periods of 35 day, 42 day, and 50 day were collected for routine blood tests, RNA extraction, and preparation of small RNA sequencing libraries. The constructed libraries were sequenced and the biological functions of differential miRNAs in RBCs in the late storage were analyzed by bioinformatics.

Results

Routine indicators of RBCs in the late stage were not significantly affected by preservation time. The Pearson correlation analysis performing on RBC miRNAs with different storage days revealed that RBC miRNAs changed with the increase of storage days. RBC miRNAs from day 35 (D35), day 42 (D42) and day 50 (D50) showed significant differences (P < 0.05). Compared RBC miRNAs from D42 with these from D35, there were 690 up-regulated miRNAs and 82 down-regulated miRNAs; compared RBC miRNAs from D50 with these from D35, there were 638 up-regulated miRNAs and 123 down-regulated miRNAs; compared RBC miRNAs from D42 with these from D50, there were 271 up-regulated miRNAs and 515 down-regulated miRNAs. GO enrichment analysis of target genes of differential miRNAs were mainly involved in cell metabolism, biosynthesis, protein modification, gene expression and transcriptional regulation of biological processes. KEGG pathway enrichment analysis of miRNA target genes showed that differential miRNA target genes were closely related to pathways in cancer.

Conclusion

MiRNAs were differentially expressed in the late stage of RBC preservation, and may be involved in various biological processes, especially cancer.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.