加强可摘局部义齿的卫生:研究用于破坏生物膜和提高抗菌效果的溶菌剂和杀菌剂。

Leonardo Guedes da Silva Moraes, Viviane de Cássia Oliveira, Ana Paula Macedo, Carolina Alves Freiria de Oliveira, Evandro Watanabe, Valéria Oliveira Pagnano
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引用次数: 0

摘要

目的:本研究评估了 2.5 mg/mL 过氧乙酸(PA)、0.5 毫克/毫升十六烷基氯化吡啶(CPC)和 160 毫克/毫升 N-乙酰半胱氨酸(NAC)对变异链球菌、金黄色葡萄球菌、白色念珠菌和白色念珠菌在热聚合丙烯酸树脂(AR)和钴铬合金(Co-Cr)表面形成的多菌种生物膜的抗生物膜作用:在丙烯酸树脂和钴铬合金试样(直径 12×3mm)表面生长多菌种生物膜。生物膜成熟后,将试样浸入实验溶液中,通过生物膜活力(CFU)(n=9)、生物膜代谢活性(XTT)(n=9)、生物膜覆盖面积(活/死)(n=2)、对胞外聚合物物质(EPS)的影响(n=2)和生物膜形态(n=1)进行评估。数据分析采用方差分析和 Tukey 后检验或 Kruskal-Wallis 后邓恩检验(α=.05):总体而言,所有评估的溶液都对生物膜的活力产生了影响。PA 具有更广泛的活性,能减少两种表面上所有微生物的 CFU,XTT(PConclusions):PA 的抗生物膜作用最大,而 CPC 和 NAC 的作用居中。不过,没有一种溶液能够完全清除附着在热聚合 AR 和 Co-Cr 合金表面的生物膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancing Removable Partial Dentures Hygiene: Investigating Mucolytic Agents and Biocides for Disrupting Biofilms and Improving Antimicrobial Efficacy.

Purpose: This study evaluates the antibiofilm action of 2.5 mg/mL peracetic acid (PA), 0.5 mg/mL cetylpyridinium chloride (CPC), and 160 mg/mL N-Acetylcysteine (NAC) against multispecies biofilm of Streptococcus mutans, Staphylococcus aureus, Candida albicans, and Candida glabrata, developed on surfaces of heat-polymerizing acrylic resin (AR) and cobaltchromium (Co-Cr) alloy.

Materials and methods: A multispecies biofilm was grown on the surface of AR and Co-Cr specimens (Ø 12×3mm). After biofilm maturation, the specimens were immersed in experimental solutions and evaluated through biofilm viability (CFU) (n=9), biofilm metabolic activity (XTT) (n=9), biofilm-covered areas (Live/Dead) (n=2), effects on the extracellular polymeric substance (EPS) (n=2) and biofilm morphology (n=1). Data were analyzed by ANOVA and the Tukey post-test or Kruskal-Wallis followed by the Dunn post-test (α=.05).

Results: Overall, all evaluated solutions impacted biofilm viability. PA presented wider activity by reducing CFU of all microorganisms on both surfaces, XTT (P<.001) and Live/Dead (P<.001). NAC had a notorious effect in reducing the viability of bacteria without affecting the yeasts. NAC reduced XTT on AR (P=.006) and Co-Cr (P=.003) but did not reduce the aggregated biofilm layer. CPC had distinct effect according to the surface, being most effective in reducing CFU on AR than the Co-Cr surface. However, it did not influence XTT, and the amount of residual aggregated biofilm.

Conclusions: PA provided the greatest antibiofilm action, while CPC and NAC showed intermediate action. Nonetheless, no solution was able to completely remove the biofilm adhered to the surfaces of heat-polymerizing AR and Co-Cr alloy.

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