与多柔比星相比,低强度二极管激光光动力疗法对 HT-29 大肠癌细胞活力的影响

IF 1.8 Q2 SURGERY
Jaber Zafari, Behnam Omidi Sarajar, Nasim Assar, Ahmad Moshaii, Emad Jafarzadeh, Fatemeh Javani Jouni
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引用次数: 0

摘要

背景和目的:结直肠腺癌被认为是其他类型恶性肿瘤中导致癌症相关死亡的主要原因之一。鉴于传统的结直肠癌治疗方案存在一些局限性和不良后果,许多研究都将重点放在了引入无害药物的新型联合方案上。本研究旨在探讨多柔比星(DOX)治疗和光动力疗法(PDT)联合使用对结直肠腺癌细胞的影响。材料与方法:HT-29 细胞分别暴露于不同浓度的 DOX、低强度(630 nm)二极管激光和作为光敏剂基质的亚甲基蓝(MB),以及它们的组合。用 3-(4,5-二甲基噻唑基-2)-2,5-二苯基溴化四氮唑(MTT)计算 DOX、激光、MB 和它们的组合的细胞毒性作用以及各处理组的 IC50 值。测定了作为脂质过氧化过程生物标志物的丙二醛(MDA)含量和上清液中释放的乳酸脱氢酶(LDH)。结果研究结果表明,与对照组和其他治疗组相比,光动力光(激光加 MB)和 DOX 的组合能显著降低 HT-29 存活细胞的百分比。此外,上述组合还大大降低了 DOX 的 IC50。培养基中 MDA 含量和 LDH 活性的显著升高分别证明了联合治疗组细胞膜脂质过氧化和细胞破坏过程的增加。结论本研究结果表明,DOX 联合光动力疗法对 HT-29 大肠腺癌细胞有更好的治疗效果。因此,在使用抗肿瘤药物的同时使用光动力疗法,可通过破坏癌细胞膜和引发死亡过程来改善癌细胞的化疗敏感性,从而减少化疗药物的所需剂量和不良反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effects of Photodynamic Therapy with Low-Level Diode Laser Compared with Doxorubicin on HT-29 Colorectal Adenocarcinoma Cells Viability.

Background and Objective: Colorectal adenocarcinoma is considered one of the major causes of cancer-related lethality among other type of malignancies. Given the several limitations and adverse outcomes of conventional therapeutic regimens against colorectal cancer, the focus of many investigations has been attributed to the introduction of a novel combined regimen with harmless agents. The purpose of the present study was to investigate the effect of combined doxorubicin (DOX) treatment and photodynamic therapy (PDT) on colorectal adenocarcinoma cells. Material and Methods: HT-29 cells were exposed to different concentrations of DOX, low-level (630 nm) diode laser, and methylene blue (MB) as a photosensitizer substrate separately and a combination of them. The cytotoxic effect of the DOX, laser, MB, and their combination and the IC50 value for each treatment group were calculated by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT). The malondialdehyde (MDA) content as a biomarker of the lipid peroxidation process and liberated lactate dehydrogenase (LDH) enzyme into supernatant was determined. Results: The results of our study evidenced that a combination of photodynamic light (laser plus MB) and DOX caused a significant reduction in the percentage of HT-29 viable cells compared with control and other treatment groups. In addition, this mentioned combination led to a considerable decrease in IC50 of DOX. Increased cell membrane lipid peroxidation and cell destruction processes in the combination therapy group were proven through significant elevation of MDA content and LDH activity in the medium, respectively. Conclusion: The findings of the present study suggested that DOX combined with PDT had a better therapeutic impact on HT-29 colorectal adenocarcinoma cells. Hence, the simultaneous application of PDT along with antineoplastic drugs improves the chemosensitivity of cancerous cells via the disruption of their membrane and triggering death processes that lead to the decrease of chemotherapeutic agents required doses and undesirable effects.

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来源期刊
CiteScore
4.10
自引率
0.00%
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0
期刊介绍: Photobiomodulation, Photomedicine, and Laser Surgery Editor-in-Chief: Michael R Hamblin, PhD Co-Editor-in-Chief: Heidi Abrahamse, PhD
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