拟南芥中 RING 型 E3 连接酶 BOI 与 EXO70E2 相互作用并介导其泛素化

IF 3.2 3区生物学 Q1 BIOLOGY

Life-Basel Pub Date : 2024-09-17 DOI:10.3390/life14091169

Zhaowu Li, Jianzhong Huang, Yue Hu, Xiaojie Zhou, Xiao Tan, Zhangying Wang, Zhiyong Gao, Xiaoqiu Wu

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引用次数: 0

摘要

外囊是一种异八聚体复合体，在调节生物过程和防御反应方面具有显著的功能多样性。在植物中，EXO70 蛋白是外囊复合体的重要组成部分，参与膜运输、生物和非生物相互作用以及细胞壁的形成。之前的一项研究表明，EXO 亚家族的一个成员 EXO70E2 与 RIN4 相互作用，介导植物免疫。在本研究中，我们发现 EXO70E2 与 RING 型 E3 连接酶 Botrytis susceptible1 interactor（BOI）相互作用，而 BOI 的 C 端结构域是其与 EXO70E2 相互作用的必要条件。此外，EXO70E2 的蛋白水平在体外被 BOI 降解和泛素化。总之，我们的研究揭示了一种通过RING型E3连接酶BOI介导泛素化来调节EXO70E2稳定性的机制。

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The RING-Type E3 Ligase BOI Interacts with EXO70E2 and Mediates Its Ubiquitination in Arabidopsis.

The exocyst is a hetero-octameric complex that exhibits significant functional diversity in regulating biological processes and defense responses. In plants, the EXO70 proteins are important components of the exocyst complex and are involved in membrane trafficking, biotic and abiotic interactions, as well as cell wall formation. A previous study has indicated that a member of the EXO subfamily, EXO70E2, interacts with RIN4 to mediate plant immunity. In this study, we found that EXO70E2 interacts with the RING-type E3 ligase Botrytis susceptible1 interactor (BOI), and the C-terminal domain of BOI is necessary for its interaction with EXO70E2. Moreover, the protein level of EXO70E2 was degraded and ubiquitinated by BOI in vitro. Collectively, our study reveals a mechanism for regulating the stability of EXO70E2 by a RING-type E3 ligase BOI-mediated ubiquitination.

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来源期刊

Life-Basel Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

CiteScore

4.30

自引率

6.20%

发文量

1798

审稿时长

11 weeks

期刊介绍： Life (ISSN 2075-1729) is an international, peer-reviewed open access journal of scientific studies related to fundamental themes in Life Sciences, especially those concerned with the origins of life and evolution of biosystems. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers.