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引用次数: 0
摘要
RNA 病毒是一种亚显微生物,其生物构成十分奇特,对人类健康有重大影响。研究 RNA 病毒的显微方法多种多样。从大到小的观察尺度依次为荧光显微镜、低温软 X 射线断层扫描(cryo-SXT)、串行低温聚焦离子束/扫描电子显微镜(cryo-FIB/SEM)体成像、低温电子断层扫描(cryo-ET)、和低温电子显微镜(cryo-EM)单粒子分析(SPA),使研究人员能够探索 RNA 病毒错综复杂的世界、其超微结构、动力学以及与宿主细胞的相互作用。这些方法结合起来可实现从原子分辨率到亚纳米分辨率的广泛分辨率范围,使相关显微技术成为一种新兴趋势。显微方法的发展提供了多方面的空间信息,促进了我们对病毒感染的了解,并为开发新型抗病毒策略和快速应对新出现的病毒威胁提供了重要工具。
Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods.
RNA viruses, being submicroscopic organisms, have intriguing biological makeups and substantially impact human health. Microscopic methods have been utilized for studying RNA viruses at a variety of scales. In order of observation scale from large to small, fluorescence microscopy, cryo-soft X-ray tomography (cryo-SXT), serial cryo-focused ion beam/scanning electron microscopy (cryo-FIB/SEM) volume imaging, cryo-electron tomography (cryo-ET), and cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) have been employed, enabling researchers to explore the intricate world of RNA viruses, their ultrastructure, dynamics, and interactions with host cells. These methods evolve to be combined to achieve a wide resolution range from atomic to sub-nano resolutions, making correlative microscopy an emerging trend. The developments in microscopic methods provide multi-fold and spatial information, advancing our understanding of viral infections and providing critical tools for developing novel antiviral strategies and rapid responses to emerging viral threats.
期刊介绍:
Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.