{"title":"模拟潜水前的二氧化碳呼吸会增加小鼠模型的减压病风险:微生物群路径不会被遗忘。","authors":"Lucille Daubresse, Aurélie Portas, Alexandrine Bertaud, Marion Marlinge, Sandrine Gaillard, Jean-Jacques Risso, Céline Ramdani, Jean-Claude Rostain, Nabil Adjiriou, Anne-Virginie Desruelle, Jean-Eric Blatteau, Régis Guieu, Nicolas Vallée","doi":"10.3390/ijerph21091141","DOIUrl":null,"url":null,"abstract":"<p><p>Decompression sickness (DCS) with neurological disorders is the leading cause of major diving accidents treated in hyperbaric chambers. Exposure to high levels of CO<sub>2</sub> during diving is a safety concern for occupational groups at risk of DCS. However, the effects of prior exposure to CO<sub>2</sub> have never been evaluated. The purpose of this study was to evaluate the effect of CO<sub>2</sub> breathing prior to a provocative dive on the occurrence of DCS in mice. Fifty mice were exposed to a maximum CO<sub>2</sub> concentration of 70 hPa, i.e., 7% at atmospheric pressure, for one hour at atmospheric pressure. Another 50 mice breathing air under similar conditions served as controls. In the AIR group (control), 22 out of 50 mice showed post-dive symptoms compared to 44 out of 50 in the CO<sub>2</sub> group (<i>p</i> < 0.001). We found that CO<sub>2</sub> breathing is associated with a decrease in body temperature in mice and that CO<sub>2</sub> exposure dramatically increases the incidence of DCS (<i>p</i> < 0.001). More unexpectedly, it appears that the lower temperature of the animals even before exposure to the accident-prone protocol leads to an unfavorable prognosis (<i>p</i> = 0.046). This study also suggests that the composition of the microbiota may influence thermogenesis and thus accidentology. Depending on prior exposure, some of the bacterial genera identified in this work could be perceived as beneficial or pathogenic.</p>","PeriodicalId":49056,"journal":{"name":"International Journal of Environmental Research and Public Health","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11431549/pdf/","citationCount":"0","resultStr":"{\"title\":\"CO<sub>2</sub> Breathing Prior to Simulated Diving Increases Decompression Sickness Risk in a Mouse Model: The Microbiota Trail Is Not Forgotten.\",\"authors\":\"Lucille Daubresse, Aurélie Portas, Alexandrine Bertaud, Marion Marlinge, Sandrine Gaillard, Jean-Jacques Risso, Céline Ramdani, Jean-Claude Rostain, Nabil Adjiriou, Anne-Virginie Desruelle, Jean-Eric Blatteau, Régis Guieu, Nicolas Vallée\",\"doi\":\"10.3390/ijerph21091141\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Decompression sickness (DCS) with neurological disorders is the leading cause of major diving accidents treated in hyperbaric chambers. Exposure to high levels of CO<sub>2</sub> during diving is a safety concern for occupational groups at risk of DCS. However, the effects of prior exposure to CO<sub>2</sub> have never been evaluated. The purpose of this study was to evaluate the effect of CO<sub>2</sub> breathing prior to a provocative dive on the occurrence of DCS in mice. Fifty mice were exposed to a maximum CO<sub>2</sub> concentration of 70 hPa, i.e., 7% at atmospheric pressure, for one hour at atmospheric pressure. Another 50 mice breathing air under similar conditions served as controls. In the AIR group (control), 22 out of 50 mice showed post-dive symptoms compared to 44 out of 50 in the CO<sub>2</sub> group (<i>p</i> < 0.001). We found that CO<sub>2</sub> breathing is associated with a decrease in body temperature in mice and that CO<sub>2</sub> exposure dramatically increases the incidence of DCS (<i>p</i> < 0.001). More unexpectedly, it appears that the lower temperature of the animals even before exposure to the accident-prone protocol leads to an unfavorable prognosis (<i>p</i> = 0.046). This study also suggests that the composition of the microbiota may influence thermogenesis and thus accidentology. Depending on prior exposure, some of the bacterial genera identified in this work could be perceived as beneficial or pathogenic.</p>\",\"PeriodicalId\":49056,\"journal\":{\"name\":\"International Journal of Environmental Research and Public Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11431549/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Environmental Research and Public Health\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.3390/ijerph21091141\",\"RegionNum\":3,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Research and Public Health","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.3390/ijerph21091141","RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
CO2 Breathing Prior to Simulated Diving Increases Decompression Sickness Risk in a Mouse Model: The Microbiota Trail Is Not Forgotten.
Decompression sickness (DCS) with neurological disorders is the leading cause of major diving accidents treated in hyperbaric chambers. Exposure to high levels of CO2 during diving is a safety concern for occupational groups at risk of DCS. However, the effects of prior exposure to CO2 have never been evaluated. The purpose of this study was to evaluate the effect of CO2 breathing prior to a provocative dive on the occurrence of DCS in mice. Fifty mice were exposed to a maximum CO2 concentration of 70 hPa, i.e., 7% at atmospheric pressure, for one hour at atmospheric pressure. Another 50 mice breathing air under similar conditions served as controls. In the AIR group (control), 22 out of 50 mice showed post-dive symptoms compared to 44 out of 50 in the CO2 group (p < 0.001). We found that CO2 breathing is associated with a decrease in body temperature in mice and that CO2 exposure dramatically increases the incidence of DCS (p < 0.001). More unexpectedly, it appears that the lower temperature of the animals even before exposure to the accident-prone protocol leads to an unfavorable prognosis (p = 0.046). This study also suggests that the composition of the microbiota may influence thermogenesis and thus accidentology. Depending on prior exposure, some of the bacterial genera identified in this work could be perceived as beneficial or pathogenic.
期刊介绍:
International Journal of Environmental Research and Public Health (IJERPH) (ISSN 1660-4601) is a peer-reviewed scientific journal that publishes original articles, critical reviews, research notes, and short communications in the interdisciplinary area of environmental health sciences and public health. It links several scientific disciplines including biology, biochemistry, biotechnology, cellular and molecular biology, chemistry, computer science, ecology, engineering, epidemiology, genetics, immunology, microbiology, oncology, pathology, pharmacology, and toxicology, in an integrated fashion, to address critical issues related to environmental quality and public health. Therefore, IJERPH focuses on the publication of scientific and technical information on the impacts of natural phenomena and anthropogenic factors on the quality of our environment, the interrelationships between environmental health and the quality of life, as well as the socio-cultural, political, economic, and legal considerations related to environmental stewardship and public health.
The 2018 IJERPH Outstanding Reviewer Award has been launched! This award acknowledge those who have generously dedicated their time to review manuscripts submitted to IJERPH. See full details at http://www.mdpi.com/journal/ijerph/awards.