热点驱动潮间带沉积物对有机和无机碳氮的吸收和短期处理

IF 3.8 1区 地球科学 Q1 LIMNOLOGY
Philip M. Riekenberg, Bradley D. Eyre, Marcel T. J. van der Meer, Joanne M. Oakes
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引用次数: 0

摘要

本研究利用双标记(13C 和 15N)稳定同位素应用来研究亚热带潮间带沉积物中微生物对有机和无机化合物中碳(C)和氮(N)的吸收和短期处理。应用了四种处理方法:(1) 藻类溶解有机物 (DOM);(2) 氨基酸混合物;(3) 葡萄糖和Ⅳ;(4) NaHCO3,并评估生物利用率对处理过程的影响(1 与 2),以及仅通过微底栖生物固定的 OM(以裙带硅藻为主)(4)与整个微生物群落吸收的物质(3)在 24 小时内的短期处理过程。24 小时后,更多来自藻类-DOM 的 13C 残留在微生物生物量中,这表明微生物利用标记的前体分子形成生物量。相反,氨基酸处理产生的 13C 没有被纳入生物量,而是被迅速呼吸为 DIC 或丢弃,因为原位微生物群落优先使用并保留了氨基酸中的 15N。葡萄糖以 CO2 形式短期输出的 13C 低于微囊底栖生物-C,而 15N 的保留在不同处理(3 和 4)之间相似,尽管施用的氮浓度增加了一倍,这表明葡萄糖可能通过硝化-反硝化作用刺激了更高的 15N 输出,但本研究未通过通量测量证实这一点。尽管对三个河口的潮汐暴露和沉积物类型进行了仔细的选址,但不同重复和不同地点对标记基质的吸收和处理存在很大差异,这对传统的统计分析提出了挑战。在微生物活动的沉积物热点地区,对基质的处理比例失调会导致数量级的差异,通过将我们的结果与之前在潮间带环境中进行的 19 项研究进行比较,发现这种差异非常普遍。开发稳健的分析技术来处理非生物和生物因素造成的变异,将使潮间带环境中的原位生物地球化学处理过程更加清晰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hot spots drive uptake and short-term processing of organic and inorganic carbon and nitrogen in intertidal sediments

This study uses dual-labeled (13C and 15N) stable isotope applications to examine microbial uptake and short-term processing of carbon (C) and nitrogen (N) from organic and inorganic compounds in subtropical intertidal sediment. Four treatment applications were applied: (1) algal dissolved organic matter (DOM), (2) amino acid mixture, (3) glucose and NH 4 + , and (4) NaHCO3 and NH 4 + to assess bioavailability effects on processing (1 vs. 2) and short-term processing for OM fixed via microphytobenthos only (pennate diatom dominated) (4) vs. material taken up by the entire microbial community (3) across 24 h. 13C from algal-DOM was preferentially used by the microbial community vs. 15N. At 24 h more 13C from algal-DOM remained in microbial biomass indicating use of labeled precursor molecules to form biomass. Conversely, 13C from the amino acid treatment was not incorporated into biomass and was either rapidly respired to DIC or discarded as the in situ microbial community preferentially used and retained 15N from amino acids. Short-term export of 13C as CO2 from glucose was lower than from microphytobenthos-C, while retention of 15N from NH 4 + was similar between treatments (3 and 4) despite doubling the application N concentration, suggesting potentially higher glucose-stimulated 15N export via nitrification–denitrification that was not confirmed via flux measurements in this study. Despite careful site selection for similar tidal exposure and sediment types among the three estuaries, the uptake and processing of labeled substrate varied substantially between replicates and sites which challenged traditional statistical analysis. Disproportionate processing of substrates occurring in sediment hotspots of microbial activity can cause variability spanning orders of magnitude which was found to be widespread through comparison of our results against 19 previous studies in intertidal settings. Development of robust analytical techniques to handle variability from abiotic and biotic factors will allow greater clarity surrounding in situ biogeochemical processing in intertidal environments.

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来源期刊
Limnology and Oceanography
Limnology and Oceanography 地学-海洋学
CiteScore
8.80
自引率
6.70%
发文量
254
审稿时长
3 months
期刊介绍: Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.
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