红茶抑制侵袭性并逆转 TNF-α 诱导的人类恶性黑色素瘤细胞侵袭性和细胞干性

IF 4.4 3区医学 Q2 ENVIRONMENTAL SCIENCES

Environmental Toxicology Pub Date : 2024-09-26 DOI:10.1002/tox.24423

Chin-Yin Lin,Shu-Chen Chu,Yih-Shou Hsieh,Wen-Yi Tsai,Pei-Ni Chen

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引用次数: 0

摘要

侵袭性和上皮-间质转化（EMT）是转移性疾病的主要模式，也是人类恶性黑色素瘤细胞与癌症相关的主要致死原因。茶叶及其提取物可降低几种癌症的患病风险，并具有抗炎和抗氧化的生物效应。然而，人们对红茶乙醇提取物（BTEE）在人类黑色素瘤中的抗EMT和抗癌干效应仍知之甚少。本研究评估了红茶乙醇提取物在人类 A 375 和 A2058 黑色素瘤细胞中降低侵袭性、EMT 和癌症干性的作用。BTEE 通过抑制 p-FAK 信号通路抑制了 u-PA 活性、迁移和侵袭性。BTEE 通过下调 β-catenin、N-cadherin、fibronectin、vimentin 和 Twist-1 的表达来影响 EMT。BTEE 还能降低肿瘤坏死因子-α（TNF-α）在体外诱导的侵袭性和癌症干性特征。裸鼠异种移植模型中黑色素瘤的生长表明，BTEE 可抑制 A 375 肿瘤在体内的生长。

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Black Tea Suppresses Invasiveness and Reverses TNF-α-Induced Invasiveness and Cell Stemness in Human Malignant Melanoma Cells.

Invasiveness and epithelial-mesenchymal transition (EMT) are main patterns of metastatic disease, which is the major cause cancer-related mortality in human malignant melanoma cells. Tea and its consumption extract are associated with a lower risk of several types of cancer and have anti-inflammatory and antioxidative biological effects. However, the anti-EMT and anti-cancer stemness effect of black tea ethanol extracts (BTEE) in human melanoma remain poorly understood. In this study, the effects of BTEE-reduced invasiveness, EMT, and cancer stemness were evaluated in human A 375 and A2058 melanoma cells. BTEE inhibited the activity of u-PA, migration, and invasiveness by repressing p-FAK signaling pathway. BTEE affected the EMT by downregulating the expression of β-catenin, N-cadherin, fibronectin, vimentin, and Twist-1. BTEE also reduced tumor necrosis factor-alpha (TNF-α)-induced invasiveness and cancer stemness characteristics in vitro. The growth of melanoma in nude mice xenograft model showed that BTEE suppressed A 375 tumor growth in vivo.

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来源期刊

Environmental Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

8.90%

发文量

261

审稿时长

4.5 months

期刊介绍： The journal publishes in the areas of toxicity and toxicology of environmental pollutants in air, dust, sediment, soil and water, and natural toxins in the environment.Of particular interest are: Toxic or biologically disruptive impacts of anthropogenic chemicals such as pharmaceuticals, industrial organics, agricultural chemicals, and by-products such as chlorinated compounds from water disinfection and waste incineration; Natural toxins and their impacts; Biotransformation and metabolism of toxigenic compounds, food chains for toxin accumulation or biodegradation; Assays of toxicity, endocrine disruption, mutagenicity, carcinogenicity, ecosystem impact and health hazard; Environmental and public health risk assessment, environmental guidelines, environmental policy for toxicants.