模拟草莓中提取的绿色碳点的多模式应用:基于槲皮素的抗氧化、抗菌和淬灭传感器

IF 7.4 2区 工程技术 Q1 ENGINEERING, CHEMICAL
Neha Sharma , Hae-Jeung Lee
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引用次数: 0

摘要

本研究利用简单的水热技术从马齿苋果实中合成了带负电荷的碳点(MSTCDs),这些碳点具有抗氧化和广谱抗菌特性,还具有检测槲皮素的特异能力。研究人员利用分光光度法(紫外可见光、荧光、X 射线光电子和傅立叶变换红外)、ZETA 电位和透射电子显微镜对 MSTCDs 进行了表征。制备的 MSTCD 平均尺寸为 8 ± 0.22 nm,zeta 电位为 -21 mV。制备的 MSTCD 的细胞毒性可忽略不计,而且与人类小胶质细胞具有生物相容性。MSTCDs 利用静态淬灭和内部滤波效应两种传感机制来检测槲皮素。MSTCDs 的荧光强度随着槲皮素剂量的增加而降低,在 0.01-70 μM 的剂量范围内呈线性相关(R2 = 0.9980),最低检测限为 2 nM。将制备的 MSTCDs 用于检测橙汁和葡萄汁中的槲皮素,结果分别为 3.27 μM 和 7.04 μM。此外,MSTCDs 还具有抗氧化活性,能清除 2,2′-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)、2,2-二苯基-1-苦基肼和羟自由基,其 EC50 值分别为 9.3、140.82 和 187.91 µg/mL。除 ABTS 自由基外,MSTCDs 的抗自由基活性略低于抗坏血酸,与对照组(EC50 = 33.7 µg/mL)相比,MSTCDs 的清除能力更强(EC50 = 9.3 µg/mL)。MSTCDs 对真菌、革兰氏阴性菌和革兰氏阳性菌具有剂量依赖性抗菌活性。因此,所开发的 CD 具有抗氧化、抗菌和淬灭槲皮素传感器的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multimodal applications of green carbon dots derived from Potentilla indica (mock strawberry): Antioxidant, antimicrobial, and quenching based quercetin sensor
This study utilized a simple hydrothermal technique to synthesize negatively charged carbon dots from Potentilla indica fruit (MSTCDs) with antioxidant and broad-spectrum antimicrobial properties, as well as a specific capacity to detect quercetin. The characterization of MSTCDs was performed using spectrophotometry (UV–vis, fluorescence, X-ray photoelectron, and Fourier-transform infrared), zeta potential, and transmission electron microscopy. The prepared MSTCDs had an average size of 8 ± 0.22 nm and zeta potential of −21 mV. The prepared MSTCDs showed negligible cytotoxicity and were found to be biocompatible with human microglial cells. MSTCDs used the static quenching and inner filter effect as their two sensing mechanisms to detect quercetin. The fluorescence intensity of the MSTCDs decreased as the dose of quercetin increased, showing a linear correlation within the dose range of 0.01–70 μM (R2 = 0.9980) and the lowest detection limit of 2 nM. The prepared MSTCDs were applied to detect quercetin in the orange and grape juice, yielding values of 3.27 and 7.04 μM, respectively. Further, the MSTCDs displayed antioxidant activity by scavenging 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl, and hydroxyl radicals, with an EC50 value of 9.3, 140.82, and 187.91 µg/mL, respectively. The antiradical activity of MSTCDs was slightly lower than the ascorbic acid except for ABTS radicals, where MSTCDs showed superior scavenging ability (EC50 = 9.3 µg/mL) compared to the control (EC50 = 33.7 µg/mL). MSTCDs exhibited dose-dependent antimicrobial activity against fungi, Gram-negative bacteria, and Gram-positive bacteria. Thus, the developed CDs showed potential as antioxidant, antimicrobial, and quenching-based quercetin sensor.
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来源期刊
Journal of Environmental Chemical Engineering
Journal of Environmental Chemical Engineering Environmental Science-Pollution
CiteScore
11.40
自引率
6.50%
发文量
2017
审稿时长
27 days
期刊介绍: The Journal of Environmental Chemical Engineering (JECE) serves as a platform for the dissemination of original and innovative research focusing on the advancement of environmentally-friendly, sustainable technologies. JECE emphasizes the transition towards a carbon-neutral circular economy and a self-sufficient bio-based economy. Topics covered include soil, water, wastewater, and air decontamination; pollution monitoring, prevention, and control; advanced analytics, sensors, impact and risk assessment methodologies in environmental chemical engineering; resource recovery (water, nutrients, materials, energy); industrial ecology; valorization of waste streams; waste management (including e-waste); climate-water-energy-food nexus; novel materials for environmental, chemical, and energy applications; sustainability and environmental safety; water digitalization, water data science, and machine learning; process integration and intensification; recent developments in green chemistry for synthesis, catalysis, and energy; and original research on contaminants of emerging concern, persistent chemicals, and priority substances, including microplastics, nanoplastics, nanomaterials, micropollutants, antimicrobial resistance genes, and emerging pathogens (viruses, bacteria, parasites) of environmental significance.
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