从一名 NF1 基因突变的 1 型神经纤维瘤病患者体内生成诱导多能干细胞系

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-09-20 DOI:10.1016/j.scr.2024.103568

Hongmei Xin , Yixiao Li , Kaihui Zhang , Aihua Ji , Meili Fan

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引用次数: 0

摘要

从携带 NF1 基因杂合突变的 1 型神经纤维瘤病（NF-1）患者身上获得了人类诱导多能干细胞（iPSC）系。外周血单核细胞（PBMC）通过非整合传递 KFL4、OCT4、SOX2、BCL-XL 和 c-MYC 进行重编程。iPSC 株系表达多能性标记，显示正常核型，并能在体外分化成三个胚层。该 iPSC 株系是人类 NF1 的重要细胞模型。

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Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation

A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.