膜蛋白供应控制烟草根细胞中原生质的生物生成

Bingqi Li, Tegan Armarego-Marriott, Łucja Kowalewska, Wolfram Thiele, Alexander Erban, Stephanie Ruf, Joachim Kopka, Mark Aurel Schöttler, Ralph Bock
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摘要

细胞色素 b559 异源二聚体是光系统 II 的一个保守成分,它在光合电子传递中的生理作用令人费解。细胞色素 b559 尤为令人费解的一点是,它存在于没有光系统 II 的幼苗中。细胞色素 b559 在蚀生体中是否具有特定功能尚不清楚。在这里,我们试图通过产生过表达 psbE 和 psbF(编码两种细胞色素 b559 蛋白的质体基因)的转质体烟草(Nicotiana tabacum)植株来解决细胞色素 b559 的功能问题。我们的研究表明,通过对启动子和翻译信号的适当处理,可以在等位质体中实现 PsbE 蛋白的过度积累,而细胞色素 b559 的水平仅有适度升高。过剩的 PsbE 蛋白会导致质粒的超微结构发生显著变化,最明显的变化是质粒体缩合,原核大量增殖,多层膜盘绕成螺旋状结构。质体脂质的分析表明,PsbE 生物合成的增加强烈刺激了质体脂质的生物合成,这表明膜蛋白的丰度控制着原胞膜的生物发生。我们的数据为 PsbE 在原绒毛体形成和原皮层膜生物生成过程中的结构作用提供了证据,并表明原皮层膜蛋白丰度调控着根瘤细胞中的脂质生物合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts
The cytochrome b559 heterodimer is a conserved component of photosystem II whose physiological role in photosynthetic electron transfer is enigmatic. A particularly puzzling aspect of cytochrome b559 has been its presence in etiolated seedlings, where photosystem II is absent. Whether or not the cytochrome has a specific function in etioplasts is unknown. Here, we have attempted to address the function of cytochrome b559 by generating transplastomic tobacco (Nicotiana tabacum) plants that overexpress psbE and psbF, the plastid genes encoding the two cytochrome b559 apoproteins. We show that strong overaccumulation of the PsbE apoprotein can be achieved in etioplasts by suitable manipulations of the promoter and the translation signals, while the cytochrome b559 level is only moderately elevated. The surplus PsbE protein causes striking ultrastructural alterations in etioplasts; most notably, it causes a condensed prolamellar body and a massive proliferation of prothylakoids, with multiple membrane layers coiled into spiral-like structures. Analysis of plastid lipids revealed that increased PsbE biosynthesis strongly stimulated plastid lipid biosynthesis, suggesting that membrane protein abundance controls prothylakoid membrane biogenesis. Our data provide evidence for a structural role of PsbE in prolamellar body formation and prothylakoid biogenesis, and indicate that thylakoid membrane protein abundance regulates lipid biosynthesis in etioplasts.
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