经处理的城市污水中的抗生素耐药大肠埃希氏菌和受水河道中的黑头鸥雏鸟

IF 4.1 2区医学 Q1 INFECTIOUS DISEASES

One Health Pub Date : 2024-09-22 DOI:10.1016/j.onehlt.2024.100901

Martina Masarikova , Iva Sukkar , Ivana Jamborova , Matej Medvecky , Ivo Papousek , Ivan Literak , Alois Cizek , Monika Dolejska

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引用次数: 0

摘要

废水是最重要的环境污染源之一，其中包括抗生素耐药细菌。这项研究的目的是评估经过处理的废水是否可能成为受纳河流中海鸥细菌定植的传播途径。研究人员采用选择性培养法收集了一批抗生素耐药大肠杆菌，它们分别来自排放到斯弗拉特卡河（捷克共和国）的经过处理的城市废水和生活在出口下游 35 公里处的黑头鸥（Chroicocephalus ridibundus）雏鸟。从总共 670 个大肠杆菌分离物（450 个来自污水，220 个来自海鸥）中，有 86 个分离物（41 个来自污水，45 个来自海鸥）显示出相同的抗生素耐药性表型和基因型，并使用脉冲场凝胶电泳（PFGE）进一步分析其克隆相关性。尽管分离物的总体多样性很高，但来自两个来源的 21 个分离物显示出相似的 PFGE 图谱。对属于流行病学重要序列类型（ST131，15 个分离株；ST23，3 个分离株）的分离株进行了全基因组测序。虽然我们的研究没有提供抗生素耐药大肠杆菌通过经处理的废水传播给野生海鸥的直接证据，但我们观察到雏鸥是多种多重耐药大肠杆菌（包括高风险克隆）的携带者，这可能会对周围环境造成进一步的细菌污染。

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Antibiotic-resistant Escherichia coli from treated municipal wastewaters and Black-headed Gull nestlings on the recipient river

Wastewaters belong among the most important sources of environmental pollution, including antibiotic-resistant bacteria. The aim of the study was to evaluate treated wastewaters as a possible transmission pathway for bacterial colonisation of gulls occupying the receiving river. A collection of antibiotic-resistant Escherichia coli originating both from treated municipal wastewaters discharged to the river Svratka (Czech Republic) and nestlings of Black-headed Gull (Chroicocephalus ridibundus) living 35 km downstream of the outlet was obtained using selective cultivation. Isolates were further characterised by various phenotyping and genotyping methods.

From a total of 670 E. coli isolates (450 from effluents, 220 from gulls), 86 isolates (41 from effluents, 45 from gulls) showed identical antibiotic resistance phenotype and genotype and were further analysed for clonal relatedness using pulsed-field gel electrophoresis (PFGE). Despite the overall high diversity of the isolates, 21 isolates from both sources showed similar PFGE profiles. Isolates belonging to epidemiologically important sequence types (ST131, 15 isolates; ST23, three isolates) were subjected to whole-genome sequencing. Subsequent phylogenetic analysis did not reveal any close clonal relationship between the isolates from the effluents and gulls' nestlings with the closest strains showing 90 SNPs difference.

Although our study did not provide direct evidence of transmission of antibiotic-resistant E. coli to wild gulls via treated wastewaters, we observed gull chicks as carriers of diverse multi-resistant E. coli, including high-risk clones, posing risk of further bacterial contamination of the surrounding environment.

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来源期刊

One Health Medicine-Infectious Diseases

CiteScore

8.10

自引率

4.00%

发文量

审稿时长

18 weeks

期刊介绍： One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.