利用 KCC-1-NH-CS2 改性导电纳米墨水对人血浆中的利伐沙班进行快速传感：一种新型生物传感器

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2024-09-21 DOI:10.1016/j.microc.2024.111744

Rokhsareh Ebrahimi , Mohammad Hasanzadeh , Nasrin Shadjou , Azadeh Nilghazi

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引用次数: 0

摘要

为检测人体血浆样本中的利伐沙班（RIV），我们开发了一种创新的纸基电化学适配体传感器。该传感器是通过在树枝状纤维状纳米二氧化硅（KCC-1-NH-CS2）颗粒上封装aptamer-aminated的新型探针制成的。然后将探针固定在功能化纳米银墨水电极上。随后，使用方波伏安法、差分脉冲伏安法和计时器评估了 prob 上产生的氧化还原反应。在优化条件下，采用无标记策略，该灵敏传感器在检测 RIV 方面表现出了适当的灵敏度和特异性，线性范围为 10 至 1000 nM，定量下限（LLOQ）为 10 nM。这种工程化的灵敏传感平台显示出巨大的潜力，可用于制造其他经济、灵敏、便携的生物传感器，用于制药和生物医学分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Aptasensing of rivaroxaban in human plasma using KCC-1-NH-CS2 modified conductive nano-ink: A new biosensor

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Aptasensing of rivaroxaban in human plasma using KCC-1-NH-CS2 modified conductive nano-ink: A new biosensor

An innovative paper-based electrochemical aptasensor has been developed for the detection of Rivaroxaban (RIV) in human plasma samples. The sensor is fabricated using a novel prob by encapsulating aptamer-aminated on dendritic fibrous nano-silica (KCC-1-NH-CS₂) particles. The prob is then immobilized on the functionalized silver nano-ink electrode.. The resulting redox reaction on the prob was subsequently evaluated using square wave voltammetry, differential pulse voltammetry, and chronoamperometry. Under optimized condition and using a label-free strategy, the aptasensor exhibited suitable sensitivity and specificity for detecting RIV with a linear range of 10 to 1000 nM and a lower limit of quantification (LLOQ) of 10 nM. The engineered aptasensing platform shows great potential for the fabrication of other cost-effective, sensitive, and portable biosensors for the pharmaceutical and biomedical analysis.

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.