Jixiang Liu , Yukun Xing , Chengkai Lv, Lan Luo, Fangfang Chen
{"title":"基于电纺碳纳米纤维的硼酸盐亲和性分子印迹电化学传感器用于选择性检测卡那霉素","authors":"Jixiang Liu , Yukun Xing , Chengkai Lv, Lan Luo, Fangfang Chen","doi":"10.1016/j.microc.2024.111743","DOIUrl":null,"url":null,"abstract":"<div><div>The presence of trace kanamycin (KANA) residues in animal-derived foods from its improper use present a critical public health issue, making the urgent need for the development of sensitive and selective KANA detection methods. In this study, we developed molecularly imprinted polymers (MIPs)-based electrochemical sensor for the detection of KANA, incorporating Co, Mo-doped carbon nanofibres (Co, Mo@CNFs) to modify a glassy carbon electrode (GCE). Then, the MIPs layer was synthesized via electropolymerization using KANA as a template molecule and 3-aminophenylboronic acid (3-APBA) as the functional monomer (as MIPs/Co, Mo@CNFs/GCE), facilitating selective recognition through boric acid affinity. The presence of Co, Mo@CNFs enhanced electron transport across the otherwise insulating MIPs layer, thereby improving the sensor’s selective recognition capabilities. Under optimal conditions, the MIPs/Co, Mo@CNFs/GCE sensor demonstrated precise KANA detection within a concentration range of 1 × 10<sup>−2</sup> to 1 × 10<sup>5</sup> nmol·L<sup>−1</sup>, achieving a detection limit of 2.56 pmol·L<sup>−1</sup>. Furthermore, the sensor’s applicability was validated in complex matrices, successfully detecting KANA in serum and milk samples with recovery rates ranging from 95.05 % to 116.43 %. These results confirmed that the proposed sensor is highly effective for detecting KANA in both biological and animal-origin food samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 111743"},"PeriodicalIF":4.9000,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Boronate affinity molecularly imprinted electrochemical sensor based on electrospun carbon nanofibres for selective kanamycin detection\",\"authors\":\"Jixiang Liu , Yukun Xing , Chengkai Lv, Lan Luo, Fangfang Chen\",\"doi\":\"10.1016/j.microc.2024.111743\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The presence of trace kanamycin (KANA) residues in animal-derived foods from its improper use present a critical public health issue, making the urgent need for the development of sensitive and selective KANA detection methods. In this study, we developed molecularly imprinted polymers (MIPs)-based electrochemical sensor for the detection of KANA, incorporating Co, Mo-doped carbon nanofibres (Co, Mo@CNFs) to modify a glassy carbon electrode (GCE). Then, the MIPs layer was synthesized via electropolymerization using KANA as a template molecule and 3-aminophenylboronic acid (3-APBA) as the functional monomer (as MIPs/Co, Mo@CNFs/GCE), facilitating selective recognition through boric acid affinity. The presence of Co, Mo@CNFs enhanced electron transport across the otherwise insulating MIPs layer, thereby improving the sensor’s selective recognition capabilities. Under optimal conditions, the MIPs/Co, Mo@CNFs/GCE sensor demonstrated precise KANA detection within a concentration range of 1 × 10<sup>−2</sup> to 1 × 10<sup>5</sup> nmol·L<sup>−1</sup>, achieving a detection limit of 2.56 pmol·L<sup>−1</sup>. Furthermore, the sensor’s applicability was validated in complex matrices, successfully detecting KANA in serum and milk samples with recovery rates ranging from 95.05 % to 116.43 %. These results confirmed that the proposed sensor is highly effective for detecting KANA in both biological and animal-origin food samples.</div></div>\",\"PeriodicalId\":391,\"journal\":{\"name\":\"Microchemical Journal\",\"volume\":\"207 \",\"pages\":\"Article 111743\"},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2024-09-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchemical Journal\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0026265X24018551\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0026265X24018551","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Boronate affinity molecularly imprinted electrochemical sensor based on electrospun carbon nanofibres for selective kanamycin detection
The presence of trace kanamycin (KANA) residues in animal-derived foods from its improper use present a critical public health issue, making the urgent need for the development of sensitive and selective KANA detection methods. In this study, we developed molecularly imprinted polymers (MIPs)-based electrochemical sensor for the detection of KANA, incorporating Co, Mo-doped carbon nanofibres (Co, Mo@CNFs) to modify a glassy carbon electrode (GCE). Then, the MIPs layer was synthesized via electropolymerization using KANA as a template molecule and 3-aminophenylboronic acid (3-APBA) as the functional monomer (as MIPs/Co, Mo@CNFs/GCE), facilitating selective recognition through boric acid affinity. The presence of Co, Mo@CNFs enhanced electron transport across the otherwise insulating MIPs layer, thereby improving the sensor’s selective recognition capabilities. Under optimal conditions, the MIPs/Co, Mo@CNFs/GCE sensor demonstrated precise KANA detection within a concentration range of 1 × 10−2 to 1 × 105 nmol·L−1, achieving a detection limit of 2.56 pmol·L−1. Furthermore, the sensor’s applicability was validated in complex matrices, successfully detecting KANA in serum and milk samples with recovery rates ranging from 95.05 % to 116.43 %. These results confirmed that the proposed sensor is highly effective for detecting KANA in both biological and animal-origin food samples.
期刊介绍:
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.