富含生物碱的 Lannea egregia 叶提取物对链脲佐菌素诱导的糖尿病大鼠的神经保护作用

Q1 Environmental Science

Toxicology Reports Pub Date : 2024-09-18 DOI:10.1016/j.toxrep.2024.101742

B.O. Ajiboye , B.E. Ekundayo , A.W. Salami , A.O. Osukoya , K. Komolafe , S. Gaur , B.E. Oyinloye , T.O. Jeje , O.A. Ojo

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引用次数: 0

摘要

背景研究表明，药用植物提取物有助于减少与糖尿病相关的神经元退化。在这项研究中，研究人员评估了叶中富含生物碱的提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠神经的保护作用。将大鼠随机分为 5 个治疗组（n = 8）：正常对照组、糖尿病诱导组（45 mg/kg STZ）、使用低剂量（50 mg/kg b.w，LEL）和高剂量（100 mg/kg b.w，LEH）（300 mg/kg 和 150 mg/kg）以及二甲双胍（200 mg/kg）治疗的糖尿病大鼠。结果与未接受治疗的糖尿病大鼠相比，接受二甲双胍、LEL 和 LEH 治疗的糖尿病大鼠的多巴胺、5-羟色胺、去甲肾上腺素、NO、MDA 水平以及 AChE 和 BChE 活性显著降低。此外，与未接受治疗的糖尿病大鼠相比，接受二甲双胍、LEL 和 LEH 治疗的糖尿病大鼠的 ENTPDase、Na/K ATPase、GST、CAT、GPx 和 SOD 活性明显提高。组织学检查显示，与糖尿病诱导的大鼠相比，治疗组的大脑结构有所改善。

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Neuroprotective effect of Lannea egregia Alkaloid-rich leaf extracts in streptozotocin-induced diabetic rats

Background

Studies suggest that medicinal plant extracts can help reduce the neuron degeneration associated with diabetes. In this study, the neuroprotective effect of the alkaloid-rich extract from the leaves of Lannea egregia was assessed in rats with diabetes induced by streptozotocin (STZ).

Methods

Lannea egregia alkaloid-rich analysis was carried out via a known procedure. The rats were randomly assigned into five treatment groups (n = 8): normal control, diabetic-induced rats (45 mg/kg STZ), and diabetic rats treated with low doses of Lannea egregia leaf alkaloid-rich extract (50 mg/kg b.w, LEL) and high (100 mg/kg b.w, LEH) (300 mg/kg and 150 mg/kg), and metformin (200 mg/kg). On 22nd day of the experiment, animals were sacrificed, and their blood and brains were collected for neuro-biomarker analysis.

Results

Diabetic-induced rats that received metformin, LEL and LEH exhibited considerably reduced levels of dopamine, serotonin, norepinephrine, NO, MDA, and AChE, BChE activities when compared to untreated diabetic animals. Additionally, rats with diabetes that received treatment with metformin, LEL and LEH displayed a noticeable increase in ENTPDase, Na/K ATPase, GST, CAT, GPx, and SOD activities when compared to the untreated diabetic rats. Histological examination revealed improved brain architecture in the treated groups in contrast to those in diabetic-induced rats.