基于协同增强过氧化物酶样活性的三维二原子纳米酶的新型酶级联比色纳米传感平台，用于检测黄嘌呤

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2024-09-21 DOI:10.1016/j.microc.2024.111735

Zichen Song , Jiayao Ou , Fengxiao Zhu , Yifan Wang , Hongda Zhu , Huiling Guo , Hongmei Sun , Mingxing Liu

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引用次数: 0

摘要

黄嘌呤（XA）水平异常可导致多种疾病，因此灵敏可靠的 XA 检测对早期诊断至关重要。本研究设计并制作了基于三维 PtAu/PCN/S-rGO 二原子纳米酶的新型酶级联比色纳米传感平台，该平台具有过氧化物酶（POD）样活性。S 和 P 的掺杂提高了电子传输速率，三维 PCN/S-rGO 载体的协同作用提高了二原子纳米酶的类过氧化物酶活性。此外，利用该纳米复合材料作为固定黄嘌呤氧化酶（XOD）的载体，构建了高效的酶级联纳米传感平台XOD@3D PtAu/PCN/S-rGO，用于XA的比色检测，可有效提高级联效率。级联反应过程中会产生中间产物-OH，在纳米复合材料的催化下，-OH氧化3,3′,5,5′-四甲基联苯胺（TMB）形成蓝色的oxTMB，使其在652 nm波长处的吸光度增加，从而产生检测XA的比色信号。XA 检测的线性范围为 1.5-250 μM，检测限为 1.29 μM。该纳米传感平台具有良好的重现性、特异性和稳定性，因此适用于检测真实人体血液样本中的 XA。因此，这种酶级联比色法硅原子纳米传感平台具有在实际临床样本中检测 XA 和其他生物标记物的潜在应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Novel enzyme cascade colorimetric nanosensing platform based on 3D diatomic nanozymes synergistically enhancing peroxidase-like activity for the detection of xanthine

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Novel enzyme cascade colorimetric nanosensing platform based on 3D diatomic nanozymes synergistically enhancing peroxidase-like activity for the detection of xanthine

Abnormal xanthine (XA) levels could cause various diseases, making sensitive and reliable XA detection crucial for early diagnosis. In this study, novel enzyme cascade colorimetric nanosensing platform based on 3D PtAu/PCN/S-rGO diatomic nanozymes with peroxidase-like (POD-like) activity was designed and fabricated. The doping of S and P increased the electron transfer rate, and the synergy of 3D PCN/S-rGO carriers increased the POD-like activity of diatomic nanozymes. Additionally, an efficient enzyme cascade nanosensing platform XOD@3D PtAu/PCN/S-rGO for the colorimetric detection of XA was constructed by utilizing this nanocomposite as a carrier for immobilizing xanthine oxidase (XOD) which could effectively enhance cascade efficiency. The intermediate •OH was produced during the cascade reaction, and the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by •OH was catalyzed by the nanocomposites to form blue oxTMB, which increased the absorbance at 652 nm and thus generated a colorimetric signal for the detection of XA. The linear range of XA detection was 1.5–250 μM, and the limit of detection was 1.29 μM. This nanosensing platform exhibited significant reproducibility, specificity, and stability, making it suitable for detecting XA in real human blood samples. Therefore, this enzyme cascade colorimetric diatomic nanosensing platform has potential applications for the detection of XA and other biomarkers in actual clinical samples.

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.