{"title":"对帕劳湖泊和瀑布中环境 DNA 的系统调查显示,洪水过后钩端螺旋体的数量有所增加","authors":"Yukuto Sato , Kaori Tsurui-Sato , Yoichiro Uchima , Cheryl-Ann Udui , Osiro Lorin , Kashgar Rengulbai , Claudia Toma , Ryo Suzuki","doi":"10.1016/j.onehlt.2024.100898","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Leptospirosis is an important bacterial zoonosis which is widespread in tropical and subtropical islands and influences human and animal health which has secondary economic effects. Although leptospirosis is endemic in Palau, an Oceanian Pacific Island country, few systematic surveys of potential risk factors for <em>Leptospira</em> infection, such as weather and host animals, have been conducted in the natural environment. We used environmental DNA metabarcoding to assess the distribution, species diversity, and abundance of pathogenic <em>Leptospira</em> in this endemic region to investigate the potential environmental risks.</div></div><div><h3>Methods</h3><div>Forty-two paired water samples, representing fine and rainy weather conditions, were collected from four representative waterfalls and lakes on Babeldaob Island, the largest island in Palau. High-throughput sequencing analysis was conducted for polymerase chain reaction products of leptospiral 16S rRNA and vertebrate animal mitochondrial 12S rRNA genes.</div></div><div><h3>Results</h3><div>We revealed greater <em>Leptospira</em> diversity and abundance in samples collected after continuous rain, particularly in the presence of flooding, compared with samples collected under typhoon, monsoon, or fine weather conditions. From same samples, six mammalian species including cats (<em>Felis catus</em>), mice (<em>Mus musculus</em>), Yap flying fox (<em>Pteropus yapensis</em>), rats (<em>Rattus spp.</em>), and pigs (<em>Sus scrofa</em>) were repeatedly detected. These may be candidates of host animals of <em>Leptospira</em> in Palau; however, their detection was not clearly correlated with that of <em>Leptospira</em>.</div></div><div><h3>Conclusion</h3><div>We repeatedly detected several species of pathogenic <em>Leptospira</em> from water samples of a wide region of Babeldaob Island. We confirmed that <em>Leptospira</em> contamination in freshwater environments increased under rainy conditions, particularly in the presence of flooding. This information could be used to improve public health control measures in this region.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100898"},"PeriodicalIF":4.1000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424002246/pdfft?md5=8156c5c891ee905e667b2cc7d2ad0451&pid=1-s2.0-S2352771424002246-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A systematic survey of environmental DNA in Palau's lakes and waterfalls reveals an increase in Leptospira levels after flooding\",\"authors\":\"Yukuto Sato , Kaori Tsurui-Sato , Yoichiro Uchima , Cheryl-Ann Udui , Osiro Lorin , Kashgar Rengulbai , Claudia Toma , Ryo Suzuki\",\"doi\":\"10.1016/j.onehlt.2024.100898\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>Leptospirosis is an important bacterial zoonosis which is widespread in tropical and subtropical islands and influences human and animal health which has secondary economic effects. Although leptospirosis is endemic in Palau, an Oceanian Pacific Island country, few systematic surveys of potential risk factors for <em>Leptospira</em> infection, such as weather and host animals, have been conducted in the natural environment. We used environmental DNA metabarcoding to assess the distribution, species diversity, and abundance of pathogenic <em>Leptospira</em> in this endemic region to investigate the potential environmental risks.</div></div><div><h3>Methods</h3><div>Forty-two paired water samples, representing fine and rainy weather conditions, were collected from four representative waterfalls and lakes on Babeldaob Island, the largest island in Palau. High-throughput sequencing analysis was conducted for polymerase chain reaction products of leptospiral 16S rRNA and vertebrate animal mitochondrial 12S rRNA genes.</div></div><div><h3>Results</h3><div>We revealed greater <em>Leptospira</em> diversity and abundance in samples collected after continuous rain, particularly in the presence of flooding, compared with samples collected under typhoon, monsoon, or fine weather conditions. From same samples, six mammalian species including cats (<em>Felis catus</em>), mice (<em>Mus musculus</em>), Yap flying fox (<em>Pteropus yapensis</em>), rats (<em>Rattus spp.</em>), and pigs (<em>Sus scrofa</em>) were repeatedly detected. These may be candidates of host animals of <em>Leptospira</em> in Palau; however, their detection was not clearly correlated with that of <em>Leptospira</em>.</div></div><div><h3>Conclusion</h3><div>We repeatedly detected several species of pathogenic <em>Leptospira</em> from water samples of a wide region of Babeldaob Island. We confirmed that <em>Leptospira</em> contamination in freshwater environments increased under rainy conditions, particularly in the presence of flooding. This information could be used to improve public health control measures in this region.</div></div>\",\"PeriodicalId\":19577,\"journal\":{\"name\":\"One Health\",\"volume\":\"19 \",\"pages\":\"Article 100898\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2352771424002246/pdfft?md5=8156c5c891ee905e667b2cc7d2ad0451&pid=1-s2.0-S2352771424002246-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"One Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352771424002246\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"One Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352771424002246","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
A systematic survey of environmental DNA in Palau's lakes and waterfalls reveals an increase in Leptospira levels after flooding
Objective
Leptospirosis is an important bacterial zoonosis which is widespread in tropical and subtropical islands and influences human and animal health which has secondary economic effects. Although leptospirosis is endemic in Palau, an Oceanian Pacific Island country, few systematic surveys of potential risk factors for Leptospira infection, such as weather and host animals, have been conducted in the natural environment. We used environmental DNA metabarcoding to assess the distribution, species diversity, and abundance of pathogenic Leptospira in this endemic region to investigate the potential environmental risks.
Methods
Forty-two paired water samples, representing fine and rainy weather conditions, were collected from four representative waterfalls and lakes on Babeldaob Island, the largest island in Palau. High-throughput sequencing analysis was conducted for polymerase chain reaction products of leptospiral 16S rRNA and vertebrate animal mitochondrial 12S rRNA genes.
Results
We revealed greater Leptospira diversity and abundance in samples collected after continuous rain, particularly in the presence of flooding, compared with samples collected under typhoon, monsoon, or fine weather conditions. From same samples, six mammalian species including cats (Felis catus), mice (Mus musculus), Yap flying fox (Pteropus yapensis), rats (Rattus spp.), and pigs (Sus scrofa) were repeatedly detected. These may be candidates of host animals of Leptospira in Palau; however, their detection was not clearly correlated with that of Leptospira.
Conclusion
We repeatedly detected several species of pathogenic Leptospira from water samples of a wide region of Babeldaob Island. We confirmed that Leptospira contamination in freshwater environments increased under rainy conditions, particularly in the presence of flooding. This information could be used to improve public health control measures in this region.
期刊介绍:
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