Julia R. Mitchell , Lindsay Vincelette , Samantha Tuberman , Vivika Sheppard , Emmett Bergeron , Roberto Calitri , Rose Clark , Caitlyn Cody , Akshara Kannan , Jack Keith , Abigail Parakoyi , MaryClare Pikus , Victoria Vance , Leena Ziane , Heather Brenhouse , Mikaela A. Laine , Rebecca M. Shansky
{"title":"雌雄大鼠各种条件性恐惧反应的行为和神经相关性","authors":"Julia R. Mitchell , Lindsay Vincelette , Samantha Tuberman , Vivika Sheppard , Emmett Bergeron , Roberto Calitri , Rose Clark , Caitlyn Cody , Akshara Kannan , Jack Keith , Abigail Parakoyi , MaryClare Pikus , Victoria Vance , Leena Ziane , Heather Brenhouse , Mikaela A. Laine , Rebecca M. Shansky","doi":"10.1016/j.ynstr.2024.100675","DOIUrl":null,"url":null,"abstract":"<div><div>Pavlovian fear conditioning is a widely used tool that models associative learning in rodents. For decades the field has used predominantly male rodents and focused on a sole conditioned fear response: freezing. However, recent work from our lab and others has identified darting as a female-biased conditioned response, characterized by an escape-like movement across a fear conditioning chamber. It is also accompanied by a behavioral phenotype: Darters reliably show decreased freezing compared to Non-darters and males and reach higher velocities in response to the foot shock (“shock response”). However, the relationship between shock response and conditioned darting is not known. This study investigated if this link is due to differences in general processing of aversive stimuli between Darters, Non-darters and males. Across a variety of modalities, including corticosterone measures, the acoustic startle test, and sensitivity to thermal pain, Darters were found not to be more reactive or sensitive to aversive stimuli, and, in some cases, they appear less reactive to Non-darters and males. Analyses of cFos activity in regions involved in pain and fear processing following fear conditioning identified discrete patterns of expression among Darters, Non-darters, and males exposed to low and high intensity foot shocks. The results from these studies further our understanding of the differences between Darters, Non-darters and males and highlight the importance of studying individual differences in fear conditioning as indicators of fear state.</div></div>","PeriodicalId":19125,"journal":{"name":"Neurobiology of Stress","volume":"33 ","pages":"Article 100675"},"PeriodicalIF":4.3000,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352289524000717/pdfft?md5=4b62059f360b89745fab62771589e0b0&pid=1-s2.0-S2352289524000717-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Behavioral and neural correlates of diverse conditioned fear responses in male and female rats\",\"authors\":\"Julia R. Mitchell , Lindsay Vincelette , Samantha Tuberman , Vivika Sheppard , Emmett Bergeron , Roberto Calitri , Rose Clark , Caitlyn Cody , Akshara Kannan , Jack Keith , Abigail Parakoyi , MaryClare Pikus , Victoria Vance , Leena Ziane , Heather Brenhouse , Mikaela A. Laine , Rebecca M. Shansky\",\"doi\":\"10.1016/j.ynstr.2024.100675\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Pavlovian fear conditioning is a widely used tool that models associative learning in rodents. For decades the field has used predominantly male rodents and focused on a sole conditioned fear response: freezing. However, recent work from our lab and others has identified darting as a female-biased conditioned response, characterized by an escape-like movement across a fear conditioning chamber. It is also accompanied by a behavioral phenotype: Darters reliably show decreased freezing compared to Non-darters and males and reach higher velocities in response to the foot shock (“shock response”). However, the relationship between shock response and conditioned darting is not known. This study investigated if this link is due to differences in general processing of aversive stimuli between Darters, Non-darters and males. Across a variety of modalities, including corticosterone measures, the acoustic startle test, and sensitivity to thermal pain, Darters were found not to be more reactive or sensitive to aversive stimuli, and, in some cases, they appear less reactive to Non-darters and males. Analyses of cFos activity in regions involved in pain and fear processing following fear conditioning identified discrete patterns of expression among Darters, Non-darters, and males exposed to low and high intensity foot shocks. 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Behavioral and neural correlates of diverse conditioned fear responses in male and female rats
Pavlovian fear conditioning is a widely used tool that models associative learning in rodents. For decades the field has used predominantly male rodents and focused on a sole conditioned fear response: freezing. However, recent work from our lab and others has identified darting as a female-biased conditioned response, characterized by an escape-like movement across a fear conditioning chamber. It is also accompanied by a behavioral phenotype: Darters reliably show decreased freezing compared to Non-darters and males and reach higher velocities in response to the foot shock (“shock response”). However, the relationship between shock response and conditioned darting is not known. This study investigated if this link is due to differences in general processing of aversive stimuli between Darters, Non-darters and males. Across a variety of modalities, including corticosterone measures, the acoustic startle test, and sensitivity to thermal pain, Darters were found not to be more reactive or sensitive to aversive stimuli, and, in some cases, they appear less reactive to Non-darters and males. Analyses of cFos activity in regions involved in pain and fear processing following fear conditioning identified discrete patterns of expression among Darters, Non-darters, and males exposed to low and high intensity foot shocks. The results from these studies further our understanding of the differences between Darters, Non-darters and males and highlight the importance of studying individual differences in fear conditioning as indicators of fear state.
期刊介绍:
Neurobiology of Stress is a multidisciplinary journal for the publication of original research and review articles on basic, translational and clinical research into stress and related disorders. It will focus on the impact of stress on the brain from cellular to behavioral functions and stress-related neuropsychiatric disorders (such as depression, trauma and anxiety). The translation of basic research findings into real-world applications will be a key aim of the journal.
Basic, translational and clinical research on the following topics as they relate to stress will be covered:
Molecular substrates and cell signaling,
Genetics and epigenetics,
Stress circuitry,
Structural and physiological plasticity,
Developmental Aspects,
Laboratory models of stress,
Neuroinflammation and pathology,
Memory and Cognition,
Motivational Processes,
Fear and Anxiety,
Stress-related neuropsychiatric disorders (including depression, PTSD, substance abuse),
Neuropsychopharmacology.