人类体外精子发生的进展：综述

IF 8.7 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Aspects of Medicine Pub Date : 2024-09-23 DOI:10.1016/j.mam.2024.101320

Anna-Lisa V. Nguyen , Sania Julian , Ninglu Weng , Ryan Flannigan

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引用次数: 0

摘要

围绕体外生精（IVS）的最新进展表明，在未来针对男性非梗阻性无精子症（NOA）的生育治疗中，体外生精有望开创一种新的再生医学模式。男性不育是一种常见病，影响着约15%的夫妇，其中15%的不育男性存在无精子症（Cocuzza等人，2013年；Esteves等人，2011年a）。对无精子症患者的治疗主要局限于手术取精结合体外受精卵胞浆内单精子显微注射（IVF-ICSI）；然而，这些患者中只有一半能成功取精，活产率通常在10%到25%之间（Aljubran等人，2022年）。IVS 被认为是进一步了解精子发生的分子和细胞过程的模型，也是一种潜在的再生治疗方法。虽然以前的研究尝试过使用人类睾丸细胞进行二维细胞培养，但由于缺乏适当的空间排列，限制了生殖细胞的分化和成熟，给临床应用带来了挑战。最近的研究表明，三维技术可以模仿人类睾丸组织的原生细胞结构，直接或间接地进行细胞间的交流，因此在 IVS 方面具有优势。三维器官型培养物、支架、器官组织、微流控、芯片睾丸和生物打印技术都显示出对再生治疗策略技术的潜在贡献，包括体外受精（IVF）。组织工程学、分子生物学和生殖医学在体外受精技术开发中的交集使得多学科的参与成为可能，在这种情况下，可以克服挑战，将再生疗法作为一种可行的选择。

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Advances in human In vitro spermatogenesis: A review

Recent advances surrounding in vitro spermatogenesis (IVS) have shown potential in creating a new paradigm of regenerative medicine in the future of fertility treatments for males experiencing non-obstructive azoospermia (NOA). Male infertility is a common condition affecting approximately 15% of couples, with azoospermia being present in 15% of infertile males (Cocuzza et al., 2013; Esteves et al., 2011a). Treatment for patients with NOA has primarily been limited to surgical sperm retrieval combined with in vitro fertilization intracytoplasmic sperm injection (IVF-ICSI); however, sperm retrieval is successful in only half of these patients, and live birth rates typically range between 10 and 25% (Aljubran et al., 2022). Therefore, a significant need exists for regenerative therapies in this patient population.

IVS has been considered as a model for further understanding the molecular and cellular processes of spermatogenesis and as a potential regenerative therapeutic approach. While 2D cell cultures using human testicular cells have been attempted in previous research, lack of proper spatial arrangement limits germ cell differentiation and maturation, posing challenges for clinical application. Recent research suggests that 3D technology may have advantages for IVS due to mimicry of the native cytoarchitecture of human testicular tissue along with cell-cell communication directly or indirectly. 3D organotypic cultures, scaffolds, organoids, microfluidics, testis-on-a-chip, and bioprinting techniques have all shown potential to contribute to the technology of regenerative treatment strategies, including in vitro fertilization (IVF).

Although promising, further work is needed to develop technology for successful, replicable, and safe IVS for humans. The intersection between tissue engineering, molecular biology, and reproductive medicine in IVS development allows for multidisciplinary involvement, where challenges can be overcome to realize regenerative therapies as a viable option.

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来源期刊

Molecular Aspects of Medicine 医学-生化与分子生物学

CiteScore

18.20

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Molecular Aspects of Medicine is a review journal that serves as an official publication of the International Union of Biochemistry and Molecular Biology. It caters to physicians and biomedical scientists and aims to bridge the gap between these two fields. The journal encourages practicing clinical scientists to contribute by providing extended reviews on the molecular aspects of a specific medical field. These articles are written in a way that appeals to both doctors who may struggle with basic science and basic scientists who may have limited awareness of clinical practice issues. The journal covers a wide range of medical topics to showcase the molecular insights gained from basic science and highlight the challenging problems that medicine presents to the scientific community.