Hongbiao Zhang, Ran Yu, Yuhao Xia, Jiawei Liu, Rong Tu, Ji Shi and Honglian Dai
{"title":"镁离子和钙离子对 GelMA/SAMA 复合水凝胶强度和生物功能性的影响。","authors":"Hongbiao Zhang, Ran Yu, Yuhao Xia, Jiawei Liu, Rong Tu, Ji Shi and Honglian Dai","doi":"10.1039/D4TB00666F","DOIUrl":null,"url":null,"abstract":"<p >Natural polymers and synthetic polymers have been extensively studied as scaffold materials, with the former offering advantages such as biocompatibility, biodegradability, and structural similarity to the natural extracellular matrix (ECM). However, the use of natural polymers in extrusion-based 3D printing has been limited by their poor mechanical properties and challenging rheological properties. In this study, gelatin and sodium alginate were utilized as scaffold materials, with the addition of Ca<small><sup>2+</sup></small> and Mg<small><sup>2+</sup></small> components to enhance their physical and chemical properties, and influence early cell behavior. Subsequently, these materials were fabricated into scaffolds using 3D printing. Our results demonstrated that the addition of Ca<small><sup>2+</sup></small> and Mg<small><sup>2+</sup></small> could improve the compactness of the 3D network structure, mechanical strength, swelling properties and degradation properties of methacrylated gelatin/methacrylated sodium alginate (GelMA/SAMA) composite hydrogel. <em>In vitro</em> cell tests revealed that the GelMA/SAMA composite hydrogel exhibited negligible cytotoxicity and promoted early cell viability, particularly with the higher concentration of Mg<small><sup>2+</sup></small> in the material. Notably, the extrusion 3D printing process successfully produced GelMA/SAMA scaffolds. These results collectively indicate that GelMA/SAMA composite scaffolds hold promise as potential biomaterials for tissue engineering applications.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 41","pages":" 10692-10704"},"PeriodicalIF":6.1000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of magnesium and calcium ions on the strength and biofunctionality of GelMA/SAMA composite hydrogels†\",\"authors\":\"Hongbiao Zhang, Ran Yu, Yuhao Xia, Jiawei Liu, Rong Tu, Ji Shi and Honglian Dai\",\"doi\":\"10.1039/D4TB00666F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Natural polymers and synthetic polymers have been extensively studied as scaffold materials, with the former offering advantages such as biocompatibility, biodegradability, and structural similarity to the natural extracellular matrix (ECM). However, the use of natural polymers in extrusion-based 3D printing has been limited by their poor mechanical properties and challenging rheological properties. In this study, gelatin and sodium alginate were utilized as scaffold materials, with the addition of Ca<small><sup>2+</sup></small> and Mg<small><sup>2+</sup></small> components to enhance their physical and chemical properties, and influence early cell behavior. Subsequently, these materials were fabricated into scaffolds using 3D printing. Our results demonstrated that the addition of Ca<small><sup>2+</sup></small> and Mg<small><sup>2+</sup></small> could improve the compactness of the 3D network structure, mechanical strength, swelling properties and degradation properties of methacrylated gelatin/methacrylated sodium alginate (GelMA/SAMA) composite hydrogel. <em>In vitro</em> cell tests revealed that the GelMA/SAMA composite hydrogel exhibited negligible cytotoxicity and promoted early cell viability, particularly with the higher concentration of Mg<small><sup>2+</sup></small> in the material. Notably, the extrusion 3D printing process successfully produced GelMA/SAMA scaffolds. These results collectively indicate that GelMA/SAMA composite scaffolds hold promise as potential biomaterials for tissue engineering applications.</p>\",\"PeriodicalId\":83,\"journal\":{\"name\":\"Journal of Materials Chemistry B\",\"volume\":\" 41\",\"pages\":\" 10692-10704\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Materials Chemistry B\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/tb/d4tb00666f\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/tb/d4tb00666f","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Effect of magnesium and calcium ions on the strength and biofunctionality of GelMA/SAMA composite hydrogels†
Natural polymers and synthetic polymers have been extensively studied as scaffold materials, with the former offering advantages such as biocompatibility, biodegradability, and structural similarity to the natural extracellular matrix (ECM). However, the use of natural polymers in extrusion-based 3D printing has been limited by their poor mechanical properties and challenging rheological properties. In this study, gelatin and sodium alginate were utilized as scaffold materials, with the addition of Ca2+ and Mg2+ components to enhance their physical and chemical properties, and influence early cell behavior. Subsequently, these materials were fabricated into scaffolds using 3D printing. Our results demonstrated that the addition of Ca2+ and Mg2+ could improve the compactness of the 3D network structure, mechanical strength, swelling properties and degradation properties of methacrylated gelatin/methacrylated sodium alginate (GelMA/SAMA) composite hydrogel. In vitro cell tests revealed that the GelMA/SAMA composite hydrogel exhibited negligible cytotoxicity and promoted early cell viability, particularly with the higher concentration of Mg2+ in the material. Notably, the extrusion 3D printing process successfully produced GelMA/SAMA scaffolds. These results collectively indicate that GelMA/SAMA composite scaffolds hold promise as potential biomaterials for tissue engineering applications.
期刊介绍:
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive:
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices