ELONGATED HYPOCOTYL5 介导的光信号促进拟南芥的嫩枝再生。

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Yu Chen, Yetkin Çaka Ince, Ayako Kawamura, David S Favero, Takamasa Suzuki, Keiko Sugimoto
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引用次数: 0

摘要

受伤的植物体细胞组织通过建立芽或根分生组织进行自我再生。在拟南芥(Arabidopsis thaliana)中,两步培养系统首先促进多能性的获得,然后指定新分生组织的命运,从而确保再生。尽管之前的研究已经报道了植物激素辅助素和细胞分裂素在决定新分生组织命运方面的重要性,但环境因素是否以及如何影响这一过程仍是未知数。在这项研究中,我们以拟南芥下胚轴为外植体,研究了光信号对芽再生的影响。我们发现,光信号在抑制根形成的同时促进了芽的再生。ELONGATED HYPOCOTYL 5(HY5)是光信号转导中的关键转录因子,通过介导控制新分生组织命运的关键基因的表达,在这一过程中发挥着核心作用。具体来说,HY5 直接抑制根系发育基因,激活芽分生组织基因,导致从多能胼胝体中建立芽原基。我们进一步证明,光合作用的早期激活对于芽的萌发至关重要,而这是由 HY5 依赖性途径的下游转录调控的。总之,我们揭示了光信号通过 HY5 调控网络控制新分生组织建立的复杂分子机制,从而突出了光信号对植物发育可塑性的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ELONGATED HYPOCOTYL5-mediated light signaling promotes shoot regeneration in Arabidopsis thaliana.

Injured plant somatic tissues regenerate themselves by establishing shoot or root meristems. In Arabidopsis (Arabidopsis thaliana), a two-step culture system ensures regeneration by first promoting the acquisition of pluripotency and subsequently specifying the fate of new meristems. Although previous studies have reported the importance of phytohormones auxin and cytokinin in determining the fate of new meristems, whether and how environmental factors influence this process remains elusive. In this study, we investigated the impact of light signals on shoot regeneration using Arabidopsis hypocotyls as explants. We found that light signals promote shoot regeneration while inhibiting root formation. ELONGATED HYPOCOTYL 5 (HY5), the pivotal transcriptional factor in light signaling, plays a central role in this process by mediating the expression of key genes controlling the fate of new meristems. Specifically, HY5 directly represses root development genes and activates shoot meristem genes, leading to the establishment of shoot progenitor from pluripotent callus. We further demonstrated that the early activation of photosynthesis is critical for shoot initiation, and this is transcriptionally regulated downstream of HY5-dependent pathways. In conclusion, we uncovered the intricate molecular mechanisms by which light signals control the establishment of new meristems through the regulatory network governed by HY5, thus highlighting the influence of light signals on plant developmental plasticity.

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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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