Viviana di Giacomo, Marwa Balaha, Morena Pinti, Maria Carmela Di Marcantonio, Ilaria Cela, Tirtha Raj Acharya, Nagendra Kumar Kaushik, Eun Ha Choi, Gabriella Mincione, Gianluca Sala, Miryam Perrucci, Marcello Locatelli, Vittoria Perrotti
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引用次数: 0
摘要
目的：冷大气等离子体（CAP）是一种治疗癌症的新方法。它可用于处理液体--等离子体活化介质（PAM），然后将其转移到靶点，作为活性氧和氮物种（RONS）的外源。本研究旨在对不同的 PAM 进行化学表征，并评估它们在体外对头颈癌细胞（HNC）的选择性：方法：将 2 毫升和 5 毫升细胞培养液分别暴露于 CAP 5 分钟、10 分钟和 20 分钟，工作距离为 6 毫米，即可获得 PAM。离子色谱法评估了阴离子动力学。通过 MTS 和流式细胞仪对人表皮角质细胞（HaCaT）和 HNC 细胞系 HSC3、HSC4 和 A253 的细胞增殖抑制、细胞凋亡发生和细胞周期改变进行评估：结果：2 mL 条件下细胞增殖明显减少，而 5 mL 条件下影响较小，但时间依赖性更明显。HaCaT 不受 5 mL PAM 的影响，这表明它对癌细胞具有选择性：通过 CAP 暴露改变的培养基化学成分通过调节细胞周期和诱导癌细胞凋亡来影响细胞增殖，而不影响正常细胞。
Cold atmospheric plasma activated media selectively affects human head and neck cancer cell lines.
Objective: Cold atmospheric plasma (CAP) is a novel approach for cancer treatment. It can be used to treat liquids-plasma-activated media (PAM)-which are then transferred to the target as an exogenous source of reactive oxygen and nitrogen species (RONS). The present study aimed at chemically characterizing different PAM and assessing their in vitro selectivity against head and neck cancer cells (HNC).
Methods: PAM were obtained by exposing 2 and 5 mL of cell culture medium to CAP for 5, 10 and 20 min at a 6 mm working distance. Anions kinetics was evaluated by ion chromatography. Cell proliferation inhibition, apoptosis occurrence, and cell cycle modifications were assessed by MTS and flow cytometry, on human epidermal keratinocyte (HaCaT) and HNC cell lines HSC3, HSC4 and A253.
Results: The 2 mL conditions showed a significant reduction in cell proliferation whereas for the 5 mL the effect was milder, but the time-dependence was more evident. HaCaT were unaffected by the 5 mL PAM, indicating a selectivity for cancer cells.
Conclusions: The media chemical composition modified by CAP exposure influenced cell proliferation by modulating cell cycle and inducing apoptosis in cancer cells, without affecting normal cells.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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