常见红葡萄(Vitis vinifera L.)种子的化学成分分析及抗氧化、抗炎、细胞毒性、镇痛和止泻活性。

IF 2.3 Q3 NUTRITION & DIETETICS
Nutrition and Metabolic Insights Pub Date : 2024-09-18 eCollection Date: 2024-01-01 DOI:10.1177/11786388241275100
Md Jamal Hossain, Khadija Rahman Lema, Md Abdus Samadd, Rumi Aktar, Mohammad A Rashid, Muhammad Abdullah Al-Mansur
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引用次数: 0

摘要

研究目的本研究旨在通过气相色谱和质谱法(GC-MS)对常见水果红葡萄(Vitis vinifera L.)种子甲醇提取物进行植物化学筛选,以确定对健康有益的生物活性化合物,并评估提取物及其馏分抗氧化、抗炎、抗疼痛和抗腹泻的药理潜力:方法:采用 DPPH 自由基清除试验、膜稳定试验和盐水虾致死生物试验,对葡萄籽甲醇提取物和各种溶剂馏分的体外抗氧化、抗炎和细胞毒性特性进行了评估。此外,研究还采用尾部浸泡法、醋酸诱导蠕动技术和蓖麻油诱导腹泻小鼠模型等方法,评估了粗提取物(200、400 和 600 毫克/千克体重)对缓解动物疼痛和减少腹泻的作用:结果:根据气相色谱-质谱分析,在种子提取物中主要发现了 73 种植物成分。在已确定的化合物中,9-十八烯酰胺(13.7%)和(9E,11E)-十八碳-9,11-二烯酸酯(11.07%)含量最高。此外,还含有几种值得注意的成分,如γ-谷甾醇、豆甾醇、副霉素、4,6-胆甾二烯醇、γ-生育三烯酚、24-亚丙基胆甾-5-烯-3beta-醇和α-生育酚乙酸酯。与标准抗氧化剂丁基化羟基甲苯(IC50 = 20.46 µg/mL)相比,葡萄籽的甲醇提取物及其不同溶剂馏分显示出良好的抗氧化特性(IC50 = 1.19-17.42 µg/mL)。与标准乙酰水杨酸(42%)相比,水溶性成分对热诱导溶血的抑制率接近 50%。此外,所有测试剂量(200、400 和 600 毫克/千克体重)的粗萃取物均显示出显著的抑制作用(P 结论:粗萃取物的抑制作用与乙酰水杨酸的抑制作用相同):目前的研究结果表明,葡萄属植物具有强大的抗氧化、抗炎、镇痛和止泻作用,因而对健康有益。不过,还需要进一步深入分析该植物的化学成分和对健康的药理作用,以便从葡萄中发现新的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical Profiling and Antioxidant, Anti-Inflammatory, Cytotoxic, Analgesic, and Antidiarrheal Activities from the Seeds of Commonly Available Red Grape (Vitis vinifera L.).

Objectives: The current study aimed to conduct a phytochemical screening of commonly known fruit red grape (Vitis vinifera L.) seed methanolic extract through gas chromatography and mass spectrometry (GC-MS) to identify the bioactive compounds responsible for its health benefits and evaluate the pharmacological potentialities of the extract and its fractions against oxidation, inflammation, pain, and diarrhea.

Methods: The in vitro antioxidant, anti-inflammatory, and cytotoxic characteristics of methanolic extracts and various solvent fractions of V. vinifera were evaluated using the DPPH free radical scavenging assay, membrane stabilizing, and brine shrimp lethality bioassay. Furthermore, the study assessed the effects of crude extracts (200, 400, and 600 mg/kg of body weight) on pain relief and reduction of diarrhea in animals using methods such as tail immersion, the acetic acid-induced writhing technique, and a diarrheal mouse model induced with castor oil.

Results: A total of 73 phytoconstituents were predominantly found in the seed extract based on the GC-MS analysis. Among the identified compounds, 9-octadecenamide (13.7%), and (9E,11E)-octadeca-9,11-dienoate (11.07%) are most abundant. Several notable constituents, such as gamma-sitosterol, stigmasterol, paromomycin, 4,6-cholestadienol, gamma-tocotrienol, 24-Propylidenecholest-5-en-3beta-ol, and alpha-tocopherol acetate, are also present. The methanolic extract of V. vinifera seed and its different solvent fractions showed promising antioxidant properties (IC50 = 1.19-17.42 µg/mL) compared to the standard antioxidant butylated hydroxytoluene (IC50 = 20.46 µg/mL). Aqueous soluble fraction exerted inhibition of nearly 50% heat-induced hemolysis compared to the standard acetylsalicylic acid (42%). Besides, all the tested doses (200, 400, and 600 mg/kg bw) of the crude extract showed significant (P < .05) analgesic and antidiarrheal effects.

Conclusion: The current findings endorsed the health benefits of V. vinifera by revealing potent antioxidant, anti-inflammatory, analgesic, and antidiarrheal effects. Nevertheless, further in-depth analysis of the plant's chemical constituents and pharmacological effects on health is warranted for novel drug discovery from V. vinifera.

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来源期刊
Nutrition and Metabolic Insights
Nutrition and Metabolic Insights NUTRITION & DIETETICS-
CiteScore
3.30
自引率
0.00%
发文量
27
审稿时长
8 weeks
期刊介绍: Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.
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