射频照射对人类神经母细胞瘤细胞中由甲萘醌诱导的 DNA 氧化损伤的保护作用:暴露持续时间的作用和对关键分子靶点的研究

IF 1.8 3区 生物学 Q3 BIOLOGY
Anna Sannino PhD, Mariateresa Allocca PhD, Maria R. Scarfì MSc, Stefania Romeo PhD, Olga Zeni PhD
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引用次数: 0

摘要

在之前的研究中,我们证明了 SH-SY5Y 人神经母细胞瘤细胞在 1950 MHz UMTS 信号(具体吸收率为 0.3 和 1.25 W/kg)下预暴露 20 小时后,能够减少随后在碱性彗星试验中使用甲萘醌处理所诱导的 DNA 氧化损伤,而本身不会诱导遗传毒性。在本研究中,使用了相同的细胞模型,通过在 72 小时培养期内设置不同的射频暴露持续时间和时间来测试相同的实验条件。至少三个独立实验的结果表明,比 20 小时更短的暴露时间,即每天 10 小时、3 小时和 1 小时,持续 3 天,仍能发挥保护作用,同时不会引起 DNA 损伤。此外,为了对所观察到的现象的机制提供一些提示,还对参与细胞应激反应的关键分子硫氧还蛋白-1、热休克转录因子 1、热休克蛋白 70 和聚[ADP-核糖]聚合酶 1 在射频暴露 3 小时后进行了 Western 印迹和 qRT-PCR 实验。在所采用的实验条件下,分子分析没有产生任何影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protective effect of radiofrequency exposure against menadione-induced oxidative DNA damage in human neuroblastoma cells: The role of exposure duration and investigation on key molecular targets

Protective effect of radiofrequency exposure against menadione-induced oxidative DNA damage in human neuroblastoma cells: The role of exposure duration and investigation on key molecular targets

In our previous studies, we demonstrated that 20 h pre-exposure of SH-SY5Y human neuroblastoma cells to 1950 MHz, UMTS signal, at specific absorption rate of 0.3 and 1.25 W/kg, was able to reduce the oxidative DNA damage induced by a subsequent treatment with menadione in the alkaline comet assay while not inducing genotoxicity per se. In this study, the same cell model was used to test the same experimental conditions by setting different radiofrequency exposure duration and timing along the 72 h culture period. The results obtained in at least three independent experiments indicate that shorter exposure durations than 20 h, that is, 10, 3, and 1 h per day for 3 days, were still capable to exert the protective effect while not inducing DNA damage per se. In addition, to provide some hints into the mechanisms underpinning the observed phenomenon, thioredoxin-1, heat shock transcription factor 1, heat shock protein 70, and poly [ADP-ribose] polymerase 1, as key molecular players involved in the cellular stress response, were tested following 3 h of radiofrequency exposure in western blot and qRT-PCR experiments. No effect resulted from molecular analysis under the experimental conditions adopted.

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来源期刊
Bioelectromagnetics
Bioelectromagnetics 生物-生物物理
CiteScore
4.60
自引率
0.00%
发文量
44
审稿时长
6-12 weeks
期刊介绍: Bioelectromagnetics is published by Wiley-Liss, Inc., for the Bioelectromagnetics Society and is the official journal of the Bioelectromagnetics Society and the European Bioelectromagnetics Association. It is a peer-reviewed, internationally circulated scientific journal that specializes in reporting original data on biological effects and applications of electromagnetic fields that range in frequency from zero hertz (static fields) to the terahertz undulations and visible light. Both experimental and clinical data are of interest to the journal''s readers as are theoretical papers or reviews that offer novel insights into or criticism of contemporary concepts and theories of field-body interactions. The Bioelectromagnetics Society, which sponsors the journal, also welcomes experimental or clinical papers on the domains of sonic and ultrasonic radiation.
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