GhCASPL1 通过稳定质膜上的纤维素合成酶复合物来调节棉纤维的次生细胞壁增厚。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Li Zhang, Xingpeng Wen, Xin Chen, Yifan Zhou, Kun Wang, Yuxian Zhu
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引用次数: 0

摘要

棉花(Gossypium hirsutum)纤维是拉长的单细胞,在次生细胞壁(SCW)增厚过程中会迅速积累纤维素,这需要纤维素合成酶复合物(CSC)的活性。在这里,我们描述了与 CSC 相互作用的因子 CASPARIAN STRIP MEMBRANE DOMAIN-LIKE1 (GhCASPL1),它通过影响 CSC 在质膜上的稳定性来促进 SCW 增厚。GhCASPL1 在 SCW 生物合成过程中优先在纤维细胞中表达,编码 MARVEL 结构域蛋白。与野生型相比,ghcaspl1 ghcaspl2 突变体的植株高度降低,产生的成熟纤维自然扭曲较少,抗拉强度较低,SCW 较细。同样,拟南芥(Arabidopsis thaliana)caspl1 caspl2 双突变体与野生型相比,茎脉管中的纤维素含量较低,细胞壁较薄,但植株形态正常。引入棉花基因 GhCASPL1 成功恢复了拟南芥 caspl1 caspl2 突变体纤维素含量的降低。洗涤剂处理、超速离心测定和酶测定显示,与野生型相比,ghcaspl1 ghcaspl2双突变体中的CSC膜结合力降低,酶活性下降。GhCASPL1 与磷脂酸(PA)结合力很强,与胚珠和叶片相比,磷脂酸在增粗纤维细胞中的含量要高得多。突变 GhCASPL1 中的 PA 结合位点会导致其失去与 GhCesA8 的共定位,并且不能定位到质膜上。PA可能会改变膜结构以促进蛋白质之间的相互作用,这表明GhCASPL1和PA共同稳定了CSC。我们的发现揭示了 CASPL 的功能以及棉纤维中 SCW 生物合成背后的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
GhCASPL1 regulates secondary cell wall thickening in cotton fibers by stabilizing the cellulose synthase complex on the plasma membrane.

Cotton (Gossypium hirsutum) fibers are elongated single cells that rapidly accumulate cellulose during secondary cell wall (SCW) thickening, which requires cellulose synthase complex (CSC) activity. Here, we describe the CSC-interacting factor CASPARIAN STRIP MEMBRANE DOMAIN-LIKE1 (GhCASPL1), which contributes to SCW thickening by influencing CSC stability on the plasma membrane. GhCASPL1 is preferentially expressed in fiber cells during SCW biosynthesis and encodes a MARVEL domain protein. The ghcaspl1 ghcaspl2 mutant exhibited reduced plant height and produced mature fibers with fewer natural twists, lower tensile strength, and a thinner SCW compared to the wild type. Similarly, the Arabidopsis (Arabidopsis thaliana) caspl1 caspl2 double mutant showed a lower cellulose content and thinner cell walls in the stem vasculature than the wild type but normal plant morphology. Introducing the cotton gene GhCASPL1 successfully restored the reduced cellulose content of the Arabidopsis caspl1 caspl2 mutant. Detergent treatments, ultracentrifugation assays, and enzymatic assays showed that the CSC in the ghcaspl1 ghcaspl2 double mutant showed reduced membrane binding and decreased enzyme activity compared to the wild type. GhCASPL1 binds strongly to phosphatidic acid (PA), which is present in much higher amounts in thickening fiber cells compared to ovules and leaves. Mutating the PA-binding site in GhCASPL1 resulted in the loss of its colocalization with GhCesA8, and it failed to localize to the plasma membrane. PA may alter membrane structure to facilitate protein-protein interactions, suggesting that GhCASPL1 and PA collaboratively stabilize the CSC. Our findings shed light on CASPL functions and the molecular machinery behind SCW biosynthesis in cotton fibers.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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